PRODUCTION OF BUTANOL BY CLOSTRIDIUM ACETOBUTYLICUM IN EXTRACTIVE FERMENTATION SYSTEM

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

SHIGEO ISHII¹  TAKESHI KOBAYASHI¹  MASAHITO TAYA¹ 

[1] Department of Chemical Engineering, Faculty of Engineering, Nagoya University

关键词: Biochemical Engineering;    Extractive Fermentation;    Butanol Production;    Aliphatic Alcohol;    Clostridium acetobutylicum;   

DOI  :  10.1252/jcej.18.125

来源: Maruzen Company Ltd

【 摘 要 】

References(20)Cited-By(68)An extractive fermentation system was developed to prevent end-product inhibition of Clostridium acetobutylicum I AM 19012, which mainly produces butanol and acetone. Butanol exhibited greater toxicity to the microorganism than acetone, and its growth was completely inhibited above 10kg/m3 of butanol. As an extracting solvent suitable for acetone-butanol fermentation, oleyl alcohol (cis-9-octadecen-l-ol) and C-20 guerbet alcohol (branched-chain alcohol of carbon number, 20) were selected from among 29 organic compounds, based on their nontoxicity to the microorganism. These two solvents had high partition coefficients for butanol, and could be reused without deterioration. In fermentation with the solvent (solvent phase: aqueous phase = 2:5 (v/v)), the viability of the microorganism was resumed by the liquid-liquid extraction of butanol from the broth, and the amount of butanol produced was 2.6 times that in fermentation without extraction.

【 授权许可】

Unknown