Nanoparticles of Selaginella doederleinii leaf extract inhibit human lung cancer cells A549

Workshop and International Seminar on Science of Complex Natural Systems

Syaefudin, S.^1 ; Juniarti, A.^1 ; Rosiyana, L.^1 ; Setyani, A.^1 ; Khodijah, S.^1

Department of Biochemistry, Bogor Agricultural University, West Java, Bogor

16680, Indonesia^1

关键词: Anticancer activities;    Cytotoxicity effects;    Effective concentration;    Encapsulants;    Human lung cancer cells;    Ionic gelations;    Leaf extracts;    Phytochemical;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/31/1/012029/pdf
DOI  :  10.1088/1755-1315/31/1/012029

来源: IOP

【 摘 要 】

The aim of the present study is to evaluate cytotoxicity effect of nanoparticles of Selaginella doederleinii (S. doederleinii) leaves extract. S. doederleinii was extracted by maceration method using 70%(v/v) ethanol as solvent. Phytochemical content was analyzed qualitatively by using Harborne and Thin Layer Chromatography (TLC) methods. Nanoparticle extract was prepared by ionic gelation using chitosan as encapsulant agent. Anticancer activity was performed by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results showed that S. doederleinii contains of flavonoids. Nanoparticle of S. doederleinii leaves extract greatly inhibited A549 cells growth (cancer cells), with IC50of 3% or 1020 μg/ml. These nanoparticles extract also inhibited the growth of Chang cells (normal cells), with IC50of 4% or 1442 μg/ml. The effective concentration of nanoparticles extract which inhibits cancer cells without harming the normal cells is 0.5% or 167 μg/ml. Further studies are needed to obtain the concentration of nanoparticles extract which can selectively suppress cancer cells.