Microfluidic Reduction of Osmotic Stress in Oocyte and Zygote Vitrification.

Microfluidics;Microfabrication;Vitrification;Assisted Reproductive Technology;Aqueous Two-Phase Systems;Rare Cell Sorting;Biomedical Engineering;Molecular;Cellular and Developmental Biology;Engineering;Health Sciences;Biomedical Engineering

Lai, DavidBull, Joseph L. ;

University of Michigan

关键词: Microfluidics;    Microfabrication;    Vitrification;    Assisted Reproductive Technology;    Aqueous Two-Phase Systems;    Rare Cell Sorting;    Biomedical Engineering;    Molecular;    Cellular and Developmental Biology;    Engineering;    Health Sciences;    Biomedical Engineering;   

Others  :  https://deepblue.lib.umich.edu/bitstream/handle/2027.42/107056/davlai_1.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Microfluidic cryoprotectant exchange enables vitrification of murine zygotes with superior morphology as indicated by a smoother cell surface and higher developmental competence compared to conventional methods. Bovine oocyte vitrification also benefit as evidenced by higher lipid retention. Experimental observations and mathematical analysis demonstrate that the microfluidic advantage arise predominantly from eliminating high shrinkage rates associated with abrupt and uneven exposure to vitrification solutions that readily occur in current manual protocols. The microfluidic cryoprotectant exchange method described has immediate applications for improving animal and human oocyte, zygote, and embryo cryopreservation. On a fundamental level, the clear demonstration that at the same minimum cell volume, cell shrinkage rate affects sub-lethal damage should be broadly useful for cryobiology.