Microtechnologies to fuel neurobiological research with nanometer precision

Journal of Nanobiotechnology

Review

Tomoyuki Kaneko¹  Fumimasa Nomura¹  Hideyuki Terazono²  Kenji Yasuda²  Ville Jokinen³  Sami Franssila³  Cecilia A Brunello⁴  Claudio Rivera⁴  Henri J Huttunen⁴  Prasanna Sakha⁵  Sari E Lauri⁵ 

[1] Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, 101-0062, Tokyo, Japan;Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, 101-0062, Tokyo, Japan;On-chip cellomics project, Kanagawa Academy of Science and Technology (KAST), 3-2-1 Sakado, Takatsu, 213-0012, Kawasaki, Japan;Department of Materials Science and Engineering, Aalto University, School Chemical Technology, Tietotie 3, FI-02150, Espoo, Finland;Neuroscience Center, University of Helsinki, P.O. Box 56, Viikinkaari 4, FI-00014, Helsinki, Finland;Neuroscience Center, University of Helsinki, P.O. Box 56, Viikinkaari 4, FI-00014, Helsinki, Finland;Department of Biosciences, Physiology, University of Helsinki, P.O. Box 65, Viikinkaari 1, FI-00014, Helsinki, Finland;

关键词: Microfluidics;    Micropatterning;    Microfabrication;    On-chip technology;    Axonal transport;    Electrophysiology;    Neurodegeneration;    Neurobiology;    Plasticity;    Connectivity;    Synaptogenesis;   

DOI  :  10.1186/1477-3155-11-11

 received in 2013-01-02, accepted in 2013-04-03,  发布年份 2013

来源: Springer

【 摘 要 】

The interface between engineering and molecular life sciences has been fertile ground for advancing our understanding of complex biological systems. Engineered microstructures offer a diverse toolbox for cellular and molecular biologists to direct the placement of cells and small organisms, and to recreate biological functions in vitro: cells can be positioned and connected in a designed fashion, and connectivity and community effects of cells studied. Because of the highly polar morphology and finely compartmentalized functions of neurons, microfabricated cell culture systems and related on-chip technologies have become an important enabling platform for studying development, function and degeneration of the nervous system at the molecular and cellular level. Here we review some of the compartmentalization techniques developed so far to highlight how high-precision control of neuronal connectivity allows new approaches for studying axonal and synaptic biology.

【 授权许可】

Unknown   
© Brunello et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.