A neuroscientist's guide to transgenic mice and other genetic tools

NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS

卷:108

Review

Navabpour, Shaghayegh¹  Kwapis, Janine L.^2,3  Jarome, Timothy J.^1,4,5 

[1] Fralin Biomed Res Inst, Translat Biol Med & Hlth, Roanoke, VA USA

[2] Penn State Univ, Dept Biol, College Pk, PA USA

[3] Penn State Univ, CMIND, College Pk, PA USA

[4] Virginia Polytech Inst & State Univ, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA

[5] Virginia Polytech Inst & State Univ, Sch Neurosci, Blacksburg, VA 24061 USA

关键词: Transgenic mice;    Knockouts;    Cre;    DREADDs;    CRISPR;    Brain;    Behavior;   

DOI  :  10.1016/j.neubiorev.2019.12.013

来源: Elsevier

【 摘 要 】

The past decade has produced an explosion in the number and variety of genetic tools available to neuroscientists, resulting in an unprecedented ability to precisely manipulate the genome and epigenome in behaving animals. However, no single resource exists that describes all of the tools available to neuroscientists. Here, we review the genetic, transgenic, and viral techniques that are currently available to probe the complex relationship between genes and cognition. Topics covered include types of traditional transgenic mouse models (knockout, knock-in, reporter lines), inducible systems (Cre-loxP, Tet-On, Tet-Off) and cell- and circuit-specific systems (TetTag, TRAP, DIO-DREADD). Additionally, we provide details on virus-mediated and siRNA/shRNA approaches, as well as a comprehensive discussion of the myriad manipulations that can be made using the CRISPR-Cas9 system, including single base pair editing and spatially- and temporally-regulated gene-specific transcriptional control. Collectively, this review will serve as a guide to assist neuroscientists in identifying and choosing the appropriate genetic tools available to study the complex relationship between the brain and behavior.

【 授权许可】

Free