The usefulness of short-term high-fat/high salt diet as a model of metabolic syndrome in mice

LIFE SCIENCES

卷:209

Article

Mendes-Junior, Leonidas Gracas¹  Freitas-Lim, Leandro Ceotto²  Oliveira, Janaina Ribeiro²  Melo, Marcos B.⁵  Feltenberger, Jonh David⁴  Brandi, Igor Viana²  Aparecida Carvalho, Bruna Mara²  Sena Guimaraes, Andre Luiz³  Batista De Paula, Alfredo Mauricio³  Mendes D'Angelis, Carlos Eduardo³  Campagnole-Santos, Maria Jose⁵  Souza Santos, Robson Augusto⁵  Braga, Valdir Andrade¹  Sousa Santos, Sergio Henrique^2,3 

[1] Univ Fed Paraiba, Dept Biotechnol, Biotechnol Ctr, Joao Pessoa, Paraiba, Brazil

[2] Univ Fed Minas Gerais, Food Engn Coll, Inst Agr Sci, Avenida Univ,1-000 Univ,39, BR-404547 Montes Claros, MG, Brazil

[3] Univ Estadual Montes Claros Unimontes, PPGCS, Lab Hlth Sci, Montes Claros, MG, Brazil

[4] Touro Univ, Sch Med, Las Vegas, NV USA

[5] Univ Fed Minas Gerais, Physiol Dept, Belo Horizonte, MG, Brazil

关键词: Metabolic syndrome;    Animal model;    Mice;    Obesity;    Heart;   

DOI  :  10.1016/j.lfs.2018.08.034

来源: Elsevier

【 摘 要 】

Diabetic cardiomyopathy (DC) describes diabetes-associated changes in the structure and function of myocardium that are not directly linked to other factors such as hypertension. Currently there are some models of DC; however, they take a large time period to mimic key features. In the present study, we investigated the effects of a short-term high-fat/high salt diet (HFHS) treatment on myocardial function and structure, and vascular reactivity in C57BL/6 male mice. After 14 weeks HFHS induced hypertension (MAP = 144.95 +/- 16.13 vs 92.90 +/- 18.95 mm Hg), low glucose tolerance (AUC = 1049.01 +/- 74.79 vs 710.50 +/- 52.57 a.u.), decreased insulin sensitivity (AUC = 429.83 +/- 35.22 vs 313.67 +/- 19.55 a.u.) and increased adiposity (epididymal fat weight 0.96 +/- 0.10 vs 0.59 +/- 0.06 OW/BW x 10(2)), aspects present in metabolic syndrome. Cardiac evaluation showed diastolic dysfunction (E/A ratio = 1.20 vs 1.90 u.a.) and cardiomyocyte hypertrophy (cardiomyocyte area = 502.82 +/- 31.46 vs 385.58 +/- 22.11 mu m(2)). Lastly, vascular reactivity was impaired with higher contractile response (136.10 +/- 3.49 vs 120.37 +/- 5.43%) and lower response to endothelium-dependent vasorelaxation (74.01 +/- 4.35 vs 104.84 +/- 3.57%). In addition, the diet was able to induce an inward coronary remodeling (vascular total area: SCNS 6185 +/- 800.6 vs HFHS 4085 +/- 213.7 mu m(2)). Therefore, we conclude that HFHS short-term treatment was able to induce metabolic syndrome-like state, cardiomyopathy and vascular injury working as an important tool to study cardiometabolic diseases.

【 授权许可】

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