Hypoglycemic effect of D-chiro-inositol in type 2 diabetes mellitus rats through the PI3K/Akt signaling pathway

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:433

Article

Gao, Yun-feng¹  Zhang, Meng-na¹  Wang, Tian-xin¹  Wu, Tian-chen¹  Ai, Ru-dan¹  Zhang, Ze-sheng¹ 

[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China

关键词: D-chiro-inositol;    Diabetes mellitus;    PI3K/Akt signaling pathway;    Liver tissue;    Skeletal muscle;   

DOI  :  10.1016/j.mce.2016.05.013

来源: Elsevier

【 摘 要 】

In this investigation, a model of type 2 diabetes mellitus (T2DM) was used on Sprague-Dawley (SD) rats to clarify more details of the mechanism in the therapy of T2DM. D-chiro-inositol (DCI) was administrated to the diabetic rats as two doses [30, 60 mg/(kg.body weight.day)]. The biochemical indices revealed that DCI had a positive effect on hypoglycemic activity and promoted the glycogen synthesis. The rats in DCI high-dosage group had a blood glucose reduction rate of 21.5% after 5 weeks of treatment, and had insulin content in serum about 15.3 +/- 2.37 mIU/L which was significantly decreased than diabetes control group. Real-time polymerase chain reaction (RT-PCR) results revealed that DCI gave a positive regulation on glycogen synthase (GS) and protein glucose transporter-4 (Glut4). Western blotting suggested that DCI could up-regulated the expression of the phosphatidylinositol-3-kinase (PI3K) p85, PI3Kp110, GS as well as the phosphorylation of protein kinase B (Akt) both in the liver and the skeletal muscle. The results also revealed that DCI enhanced the Glut4 expression on skeletal muscle. Above all, DCI played a positive role in regulating insulin-mediated glucose uptake through the PI3K/Akt signaling pathway in T2DM rats. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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