Differential Gene Expression and Biological Analyses of Primary Hepatocytes Following D-Chiro-Inositol Supplement

Frontiers in Endocrinology

Feier Cheng¹  Shao-jun Yun¹  Jing-yu Liu¹  Cui-ping Feng¹  Jin-ling Cao¹  Yan-fen Cheng¹  Ming-chang Chang²  Jun-long Meng² 

[1] College of Food Science and Engineering, Shanxi Agricultural University, Taigu, China;Shanxi Research Station for Engineering Technology of Edible Fungi, Shanxi Agricultural University, Taigu, China;

关键词: primary hepatocyte;    D-chiro-inositol;    RNA‐Seq;    insulin resistance;    molecule mechanism;   

DOI  :  10.3389/fendo.2021.700049

来源: DOAJ

【 摘 要 】

Dietary supplements have improved the prevention of insulin resistance and metabolic diseases, which became a research hotspot in food science and nutrition. Obesity and insulin resistance, caused by a high-fat diet, eventually result in severe metabolic diseases, can be prevented with the dietary supplement D-chiro-inositol (DCI). In this work, we isolated mice primary hepatocytes with palmitic acid stimulation and DCI was applied to compare and contrast its effects of in primary hepatocyte biology. Before and after intervention with DCI, we used RNA-Seq technology to establish a primary hepatocyte transcriptome gene profile. We found that both PA and DCI cause a wide variation in gene expression. Particularly, we found that DCI plays critical role in this model by acting on glycolysis and gluconeogenesis. Overall, we generated extensive transcripts from primary hepatocytes and uncovered new functions and gene targets for DCI.

【 授权许可】

Unknown