Label-free electrochemical aptasensor for the detection of lysozyme

TALANTA

卷:78

Article

Rodriguez, Marcela C.¹  Rivas, Gustavo A.¹ 

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Fis, INFIQC, RA-5000 Cordoba, Argentina

关键词: Aptamer;    Carbon paste electrode;    Lysozyme;    Electrochemical aptasensor;    Protein detection;    Biosensor;   

DOI  :  10.1016/j.talanta.2008.11.002

来源: Elsevier

【 摘 要 】

This work reports the advantages of a label free electrochemical aptasensor for the detection of lysozyme. The biorecognition platform was obtained by the adsorption of the aptamer on the surface of a carbon paste electrode (CPE) previously blocked with mouse immunoglobulin under controlled-potential conditions. The recognition event was detected from the decrease in the guanine and adenine electro-oxidation signals produced as a consequence of the molecular interaction between the aptamer and lysozyme. The biosensing platform demonstrated to be highly selective even in the presence of large excess (9-fold) of bovine serum albumin, cytochrome C and myoglobin. The reproducibility for 10 repetitive determinations of 10.0 mg L-1 lysozyme solution was 5.1% and 6.8% for guanine and adenine electro-oxidation signals, respectively. The detection limits of the aptasensor were 36.0 nmol L-1 (if considering guanine signal) and 18.0 nmol L-1 (if taking adenine oxidation current). This new sensing approach represents an interesting and promising alternative for the electrochemical quantification of lysozyme. (c) 2008 Elsevier B.V. All rights reserved.

【 授权许可】

Free