Detection of vitamin b1 (thiamine) using modified carbon paste electrodes with polypyrrole

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Muppariqoh, N.M.^1 ; Wahyuni, W.T.^1,2 ; Putra, B.R.^1

Chemistry Department, Bogor Agricultural University, Bogor, Indonesia^1

Pusat Studi Biofarmaka Tropika, Lembaga Penelitian Dan Pengabdian Kepada Masyarakat, Bogor, Indonesia^2

关键词: Alkaline medium;    Carbon paste electrode;    Detection limits;    Modified carbon paste electrode;    Molecularly Imprinted Polymer;    Optimum conditions;    Quantitation limits;    Working electrode;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012050/pdf
DOI  :  10.1088/1755-1315/58/1/012050

来源: IOP

【 摘 要 】

Vitamin B1(thiamine) is oxidized in alkaline medium and can be detected by cyclic voltammetry technique using carbon paste electrode (CPE) as a working electrode. polypyrrole-modified CPE were used in this study to increase sensitivity and selectivity measurement of thiamine. Molecularly imprinted polymers (MIP) of the modified CPE was prepared through electrodeposition of pyrrole. Measurement of thiamine performed in KCl 0.05 M (pH 10, tris buffer) using CPE and the modified CPE gave an optimum condition anodic current of thiamine at 0.3 V, potential range (-1.6-1 V), and scan rate of 100 mV/s. Measurement of thiamine using polypyrrole modified CPE (CPE-MIPpy) showed better result than CPE itself with detection limit of 6.910-5M and quantitation limit 2.110-4M. CPE-MIPpy is selective to vita min B1. In conclusion, CPE-MIPpy as a working electrode showed better performance of thiamine measurement than that of CPE.