Tumor-targeting aptamers for the treatment of prostate cancer.

Aptamer;Cancer stem cell;Chemotherapy;Prostate cancer

Enid W. Choi

University:University of Louisville

Department:Biochemistry and Molecular Biology

关键词: Aptamer;    Cancer stem cell;    Chemotherapy;    Prostate cancer;   

Others  :  https://ir.library.louisville.edu/cgi/viewcontent.cgi?article=1244&context=etd

美国|英语

来源: The Universite of Louisville's Institutional Repository

【 摘 要 】

Prostate cancer is the most common type diagnosed among men in the United States.Random oligodeoxynucleotide (ODN) libraries are used to generate DNA aptamers bysystematic evolution of ligands by exponential enrichment (SELEX). We explored theutility of guanine-rich libraries, and found that some had cancer-selective antiproliferativeactivity that generally correlated with G-quadruplex formation, nuclease resistance, andenhanced cellular uptake. We identified several proteins by mass spectrometry that bindto GC and cytosine ODNs. We compared the GT library with the classic random libraryfor SELEX of whole prostate cancer cells and identified a new aptamer from the GTlibrary with enhanced nuclease resistance and antiproliferative activity. We presentevidence that ASI411, a known G-rich anticancer aptamer, inhibits prostate tumorinitiating cells. This body of work supports the hypothesis that G-rich ODNs are wellsuited to the development of new aptamers in the treatment of cancer.