Biologically friendly room temperature ionic liquids and nanomaterials for the development of innovative enzymatic biosensors: Part II

TALANTA

卷:194

Article

Zappi, Daniele¹  Gabriele, Serena¹  Gontrani, Lorenzo²  Dini, Danilo¹  Sadun, Claudia¹  Marini, Federico¹  Antonelli, Marta Letizia¹ 

[1] Univ Roma La Sapienza, Dept Chem, Ple A Moro 5, I-00185 Rome, Italy

[2] Univ Bologna, Dept Chem Giacomo Ciamician, VF Selmi 2, I-40126 Bologna, Italy

关键词: Enzymatic biosensor;    Ionic liquid;    Nanostructured SPE;    Alcohol dehydrogenase;    Lipase;    EVOO;   

DOI  :  10.1016/j.talanta.2018.10.001

来源: Elsevier

【 摘 要 】

A newly modified electrode based on glassy carbon (GC) has been prepared and characterized electrochemically for application in electroanalytical chemistry. In particular, a GC screen-printed electrode (SPE) has been modified with nanostructures, namely multi-walled carbon nanotubes (MWCNTs), and TiO2 nanoparticles, and combined with a new generation of eco-friendly room-temperature ionic liquids (RTILs). The green RTILs here used are suitable for the immobilization of enzymes on the electrode surface and, additionally, facilitate the kinetics of electron transfer due to their intrinsic electrical conductivity. Upon evaluation of these newly modified electrodes we found an improvement in terms of electrochemically active area (Aea) with respect to the electrodes we previously reported. The modified SPEs were then used as substrates for the construction of two enzymatic biosensors for analytical applications: the first is an enzymatic biosensor based on alcohol dehydrogenase (ADH) for the analysis of ethyl alcohol; the second biosensor is based on lipase enzyme and has been tested for the analysis and the classification of Extra Virgin Olive Oil (EVOO). The performances of the here projected sensors appear comparable with biosensors having similar finalities. It is here envisaged that such a kind of electrodes could represent the starting tool for the construction and the definition of new portable devices for screening and field analyses.

【 授权许可】

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