Molinate quantification in environmental water by a glutathione-S-transferase based biosensor

TALANTA

卷:106

Article

Oliveira, Tulio I. S.^1,2  Oliveira, Marcela^1,3  Viswanathan, Subramanian¹  Fatima Barroso, M.¹  Barreiros, Luisa⁴  Nunes, Olga C.⁴  Rodrigues, Jose A.³  de Lima-Neto, Pedro²  Mazzetto, Selma E.²  Morais, Simone¹  Delerue-Matos, Cristina¹ 

[1] Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE, P-4200072 Oporto, Portugal

[2] Univ Fed Ceara, Ctr Ciencias, Dept Quim Organ & Inorgan, BR-60455970 Fortaleza, Ceara, Brazil

[3] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE, P-4169007 Oporto, Portugal

[4] Univ Porto, Fac Engn, Dept Engn Quim, LEPAE, P-4200465 Oporto, Portugal

关键词: Glutathione-S-transferase;    Enzymatic biosensor;    Voltammetry;    Molinate;    Rice paddy field floodwater;   

DOI  :  10.1016/j.talanta.2012.10.074

来源: Elsevier

【 摘 要 】

A glutathione-S-transferase (GST) based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water. The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane-glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mg L-1 and presenting a detection limit of 0.064 mg L-1. The developed biosensor is stable, and reusable during 15 days. The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements. (C) 2012 Elsevier B.V. All rights reserved.

【 授权许可】

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