A new method for the determination of primary and secondary terrestrial and marine biomarkers in ice cores using liquid chromatography high-resolution mass spectrometry

TALANTA

卷:194

Article

King, Amy C. F.¹  Giorio, Chiara^2,5  Wolff, Eric³  Thomas, Elizabeth¹  Karroca, Ornela^2,4  Roverso, Marco⁵  Schwikowski, Margit⁶  Tapparo, Andrea⁵  Gambaro, Andrea⁴  Kalberer, Markus² 

[1] British Antarctic Survey, Madingley Rd, Cambridge CB3 0ET, England

[2] Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England

[3] Univ Cambridge, Dept Earth Sci, Downing St, Cambridge CB2 3EQ, England

[4] Ca Foscari Univ Venice, Venice, Italy

[5] Univ Padua, Dipartimento Sci Chim, Via Marzolo 1, I-35131 Padua, Italy

[6] Paul Scherrer Inst, Forschwngsstr 111, CH-5232 Villigen, Switzerland

关键词: Ice core;    Organic aerosol;    Biomarker;    Mass spectrometry;    Rotary evaporation;    Paleoclimate;   

DOI  :  10.1016/j.talanta.2018.10.042

来源: Elsevier

【 摘 要 】

The majority of atmospheric compounds measured in ice cores are inorganic, while analysis of their organic counterparts is a less well developed field. In recent years, understanding of formation, transport pathways and preservation of these compounds in ice and snow has improved, showing great potential for their use as biomarkers in ice cores. This study presents an optimised analytical technique for quantification of terrestrial and marine biosphere emissions of secondary organic aerosol (SOA) components and fatty acids in ice using HPLC-MS analysis. Concentrations of organic compounds in snow and ice are extremely low (typically ppb or ppt levels) and thus pre-concentration is required prior to analysis. Stir bar sorptive extraction (SBSE) showed potential for fatty acid compounds, but failed to recover SOA compounds. Solid phase extraction (SPE) recovered compounds across both organic groups but methods improving some recoveries came at the expense of others, and background contamination of fatty acids was high. Rotary evaporation was by far the best performing method across both SOA and fatty acid compounds, with average recoveries of 80%. The optimised pre-concentration - HPLC-MS method achieved repeatability of 9% averaged for all compounds. In environmental samples, both concentrations and seasonal trends were observed to be reproducible when analysed in two different laboratories using the same method.

【 授权许可】

Free