Versatile digital polymerase chain reaction chip design, fabrication, and image processing

SENSORS AND ACTUATORS B-CHEMICAL

卷:283

Article

Li, Huanan¹  Zhang, Haoqing¹  Xu, Ying¹  Tureckova, Alzbeta^1,2  Zahradnik, Pavel^1,3  Chang, Honglong¹  Neuzil, Pavel^1,4 

[1] Northwestern Polytech Univ, Dept Microsyst, Fac Mech Engn, 127 West Youyi Rd, Xian 710072, Shaanxi, Peoples R China

[2] Tomas Bata Univ Zlin, Fac Appl Informat, Nam TG Masaryka 5555, Zlin 76001, Czech Republic

[3] Czech Tech Univ, Fac Elect Engn, Dept Telecommun Engn, Tech 1902 2, Prague 16627, Czech Republic

[4] Brno Univ Technol, Fac Elect Engn & Commun, SIX Ctr, Dept Microelect,Tech 3058 10, Brno 61600, Czech Republic

关键词: Digital PCR;    Silicon chip;    Fluorescence image analysis;    DNA counting;    Microfluidic chip;    Array;   

DOI  :  10.1016/j.snb.2018.12.072

来源: Elsevier

【 摘 要 】

We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 x 9) mm(2) in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 x 2785 mu m(2) that contain reaction wells with a gap of a few mu m separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells' positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.

【 授权许可】

Free