INTEGRATING DROPLET MICROFLUIDICS AND CONFOCAL FLUORESCENCE SPECTROSCOPY FOR HIGH-THROUGHPUT BIOMOLECULAR ANALYSIS

Droplets;Microfluidics;Confocal Fluorescence Spectroscopy;Digital PCR;LAMP;High-throughput screening;Biomedical Engineering

Rane, Tushar D.Mao, Hai-Quan ;

Johns Hopkins University

关键词: Droplets;    Microfluidics;    Confocal Fluorescence Spectroscopy;    Digital PCR;    LAMP;    High-throughput screening;    Biomedical Engineering;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/40183/Supp_video1.wmv?sequence=8&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

Droplet microfluidics has emerged as a branch of microfluidics with tremendous potential for high-sensitivity and high-throughput biomolecular analysis, leading to its adaptation for a variety of applications. However, commonly used biosample analysis schemes using droplet-based systems are limited, due to the need for multiple devices per analysis, integration with low-sensitivity detection techniques as well as the need of barcodes for droplet identification. This thesis will focus on sample-to-answer droplet microfluidic devices I have developed in collaboration with colleagues at the Johns Hopkins University to overcome these limitations. Integration of novel microfluidic design with highly sensitive confocal fluorescence spectroscopy technique has enabled these devices to achieve ultra-sensitive analysis of individual biosamples at single-molecule levels as well as high throughput multi-analyte analysis of biosamples.