Direct and label-free monitoring oligonucleotide immobilization, non-specific binding and DNA biorecognition

SENSORS AND ACTUATORS B-CHEMICAL

卷:192

Article

Luis Lopez-Paz, Jose¹  Angel Gonzalez-Martinez, Miguel¹  Escorihuela, Jorge¹  Banuls, Maria-Jose¹  Puchades, Rosa¹  Maquieira, Angel¹ 

[1] Univ Politecn Valencia, Ctr Reconocimiento Mol & Desarrollo Tecnol, Dept Quim, Valencia 46022, Spain

关键词: DNA immobilization;    Silicon;    Surface chemistry;    Dual Polarization Interferometry;    Biosensing;   

DOI  :  10.1016/j.snb.2013.10.110

来源: Elsevier

【 摘 要 】

DNA binding chemistry on silicon surface has been investigated. Aminated oligonucleotide probes were immobilized on the chip surface by chemical silanization and further covalent attachment. The chemistries employed were the classical 3-aminopropyltriethoxysilane/glutaraldehyde and, for comparison purposes, the novel 3-isocyanatepropyltriethoxysilane, that allows the direct attachment of the aminated probe. Alternatively, a thiolated oligonucleotide was also photochemically immobilized by means of a thioether linkage. The experiments were followed label-free by Dual Polarization Interferometry. All chemical and photochemical methods gave rise to a probe density immobilization in the order of 1.0-2.5 x 10(10) molecules/mm(2), similar to the values reported for other chemistries. The obtained data suggest that DNA strands are anchored in a different conformation depending on the immobilization method employed. In order to avoid non-specific binding of target molecules, ethanolamine and inert proteins were assayed, and successful results were obtained when using small size proteins such as gelatine. Hybridization efficiency was around 20% for aminosilane-based immobilized probes, and more than 4-fold this value when the other immobilization methods were employed. The ability for recognition complementary DNA strands discriminating non-complementary ones was applied for species identification in mixtures. (C) 2013 Elsevier B.V. All rights reserved.

【 授权许可】

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