Ultrahigh-sensitive wireless QCM with bio-nanocapsules

SENSORS AND ACTUATORS B-CHEMICAL

卷:293

Article

Noi, Kentaro¹  Iijima, Masumi^2,3  Kuroda, Shun'ichi³  Ogi, Hirotsugu¹ 

[1] Osaka Univ, Grad Sch Engn, Yamadaoka 2-1, Suita, Osaka 5650871, Japan

[2] Tokyo Univ Agr, Fac Appl Biosci, Setagaya Ku, Tokyo 1568502, Japan

[3] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

关键词: QCM;    Bio-nanocapsule;    Multichannel;    Wireless;    CRP;   

DOI  :  10.1016/j.snb.2019.04.150

来源: Elsevier

【 摘 要 】

Quartz-crystal-microbalance (QCM) biosensor is a mass-sensitive biosensor, and its sensitivity in detecting target proteins can be improved with weighted detection antibodies. In this communication, we propose a mass-amplified detection of biomarkers by bio-nanocapsules (BNCs) in wireless high-frequency multichannel QCM biosensor. BNC exhibits molecular mass of 6.57 MDa and possesses many immunoglobulin-G (IgG) Fc-binding Z domains on its surface, making it possible to display similar to 60 antibodies in an oriented immobilization manner. We find that by reinforcing the bond strength between the Z domain and the Fc part of antibody with the cross-linker, a high sensitive detection of biomarkers with QCM is realized. We used multichanel wireless QCM system with fundamental resonance frequencies above 54 MHz and performed detection of C-reactive protein. The detection limit is lower than 10 pg/mL, confirming that combination of BNC and wireless high-frequency QCM allows high sensitivity detection of biomarkers.

【 授权许可】

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