A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway

Journal of Nanobiotechnology

Short Communication

Koichi Takeda¹  Ryosuke Ezawa¹  Chiaki Ogino¹  Akihiko Kondo¹  Yuya Nishimura²  Jun Ishii² 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Japan;Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Japan;

关键词: Bio-nanocapsule;    pH-sensitive peptide;    GALA;    Endosomal escape;    HER2;    Drug delivery;   

DOI  :  10.1186/1477-3155-12-11

 received in 2013-11-13, accepted in 2014-03-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAn affibody-displaying bio-nanocapsule (ZHER2-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, ZHER2, that recognizes HER2 receptors. This affibody was previously reported to be the result of the endocytosis-dependent specific uptake of proteins and siRNA into target cancer cells. To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a ZHER2-BNC.FindingsIn this study, we displayed a pH-sensitive fusogenic GALA peptide on the surface of a particle in order to confer the ability of endosomal escape to a ZHER2-BNC. A GALA-displaying ZHER2-BNC purified from yeast uneventfully formed a particle structure. Furthermore, endosomal escape of the particle was facilitated after endocytic uptake and release of the inclusions to the cytoplasm without the cell toxicity.ConclusionThe genetic fusion of a GALA peptide to the virus-like particle confers the ability of endosomal escape.

【 授权许可】

Unknown   
© Nishimura et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.