Detection of atherosclerosis-associated HOCl using a mitochondria-targeted fluorescent probe

SENSORS AND ACTUATORS B-CHEMICAL

卷:348

Article

Wang, Bingya^1,5  Yuan, Fengying²  Wang, Shan⁵  Ren, Wen Xiu^2,3  Hou, Ji-Ting^1,4,5 

[1] Wenzhou Med Univ, Sch Ophthalmol & Optometry, Sch Biomed Engn, Wenzhou 325035, Zhejiang, Peoples R China

[2] Southwest Med Univ, Dept Radiol, Affiliated Hosp, Luzhou 646000, Peoples R China

[3] Southwest Med Univ, Affiliated Hosp, Nucl Med & Mol Imaging Key Lab Sichuan Prov, Luzhou 646000, Peoples R China

[4] Hainan Med Univ, Coll Emergency & Trauma, Key Lab Hainan Trauma & Disaster Rescue, Key Lab Emergency & Trauma,Minist Educ, Haikou 571199, Hainan, Peoples R China

[5] Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China

关键词: Fluorescent probe;    Mitochondria;    Hypochlorous acid;    Atherosclerosis;    Foam cells;   

DOI  :  10.1016/j.snb.2021.130695

来源: Elsevier

【 摘 要 】

Atherosclerosis is closely associated with cardiovascular and cerebrovascular diseases, which are the leading causes of death worldwide. The early diagnosis of atherosclerosis is helpful for prevention and treatment of related diseases. The formation of foam cells with upregulated HOCl level has been considered as a hallmark of the early atherosclerosis lesion. In this work, we reported a fluorescent probe AS-ClO for the detection of mitochondrial HOCl in foam cells for the first time. Upon reaction with HOCl, AS-ClO produced a dramatic fluorescence enhancement at 520 nm. The probe showed excellent selectivity toward HOCl with a low detection limit (12.53 nM) and a rapid response (within 5s). The mitochondria targetability of the probe was further confirmed by co-imaging test. The foam cells derived from MOVAS and RAW264.7 cells pre-treated with oxidized low-density lipoprotein exhibited 12.58-fold and 10.88-fold fluorescence enhancement with the help of the proposed probe, respectively. Moreover, a higher mitochondrial HOCl level in calcified MOVAS cells was imaged for the first time. Lastly, the over-production of HOCl in abdominal aorta sections from atherosclerotic mice was observed. The probe is anticipated to be used in the diagnosis of atherosclerosis and related diseases.

【 授权许可】

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