A mitochondria-specific fluorescent probe for rapidly assessing cell viability

TALANTA

卷:221

Article

Chen, Jianmei¹  Xu, Yurui¹  Gao, Ya¹  Sun, Lei¹  Meng, Xia¹  Gu, Kai²  Zhang, Yu¹  Ning, Xinghai¹ 

[1] Nanjing Univ, Coll Engn & Appl Sci, Jiangsu Key Lab Artificial Funct Mat, Natl Lab Solid State Microstruct,Chem & Biomed In, Nanjing 210093, Peoples R China

[2] China Pharmaceut Univ, Coll Med, State Key Lab Nat Med, 24 Tongjiaxiang, Nanjing 210009, Peoples R China

关键词: Mitochondria;    Fluorescent probe;    High specificity;    Cell viability;    Drug screening;   

DOI  :  10.1016/j.talanta.2020.121653

来源: Elsevier

【 摘 要 】

Rapid detection of cell viability is of importance for various aspects of basic and applied research. However, the most commonly used MTT assay suffers from some disadvantages of time-consuming, poor solubility of formazan crystals, and interference of cell extracts, resulting in either delayed results or inconsistent cell viability. Accumulating evidences show that mitochondrial activity is closely associated with cell proliferation and apoptosis, making it valuable for real-time monitoring of cell fates. Herein, we present a novel type of cell-permeant mitochondrial dyes, composed of coumarin-quinoline conjugates (CQCs), for assessing cell viability through monitoring mitochondrial dynamic changes. CQCs exhibited superior preference for mitochondria, and could sensitively monitor their fissions under pathological conditions. Particularly, CQCs could accurately assess the cell viability within 10 min, allowing for rapidly evaluating therapeutic effects with high sensitivity and accuracy, in comparison of MTT assay. Therefore, CQCs provide a proof-of-concept assay that is appropriately practical either independently or in complementation with other assays for the analysis of cellular viability in broad range of biomedical applications.

【 授权许可】

Free