Hypochlorite modified LDL are a stronger agonist for platelets than copper oxidized LDL

FEBS Letters

Volf, Ivo¹  Koller, Elisabeth¹  Roth, Astrid¹  Cooper, Julian¹  Moeslinger, Thomas¹ 

[1] Institute of Physiology, Schwarzspanierstr. 17, A-1090 Vienna, Austria

关键词: Oxidized low density lipoprotein;    Hypochlorous acid;    Trace metal;    Platelet aggregation;    Atherosclerosis;    Thrombosis;    LDL;    low density lipoprotein;    OxLDL;    oxidized low density lipoprotein;    M-HSA;    maleylated human serum albumin;    hyp-OxLDL;    low density lipoprotein oxidized by HOCl/OCl−;    Cu-OxLDL;    copper oxidized low density lipoprotein;    ADP;    adenosine 5′-diphosphate;    apoB;    apolipoprotein B-100;    f.c.;    final concentration;   

DOI  :  10.1016/S0014-5793(00)02104-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experimental low density lipoprotein (LDL) oxidation is usually performed using trace copper, although the in vivo relevance of this method has been called into question. Such LDL augment adenosine 5′-diphosphate (ADP) induced platelet aggregation, presumably by the action of lipid derived compounds. In striking contrast, we find that LDL oxidized to a comparable extent by hypochlorite, an in vivo occurring oxidant, reveal themselves to be potent promoters of platelet aggregation. Interestingly, hypochlorite modified LDL seem to mediate their influence on human platelets by means of the modified apolipoprotein B-100 (apoB) moiety. Also, the finding that hypochlorite modified albumin is able to trigger platelet aggregation suggests an essential role for hypochlorite modified protein(s) in the process of platelet activation.

【 授权许可】

Unknown