Sustained neuronal and microglial alterations are associated with diverse neurobehavioral dysfunction long after experimental brain injury

NEUROBIOLOGY OF DISEASE

卷:136

Article

Ritzel, Rodney M.^1,2  Li, Yun^1,2  He, Junyun^1,2  Khan, Niaz^1,2  Doran, Sarah J.^1,2  Faden, Alan, I^1,2,3  Wu, Junfang^1,2,3 

[1] Univ Maryland, Sch Med, Dept Anesthesiol, Baltimore, MD 21201 USA

[2] Univ Maryland, Sch Med, Ctr Shock Trauma & Anesthesiol Res STAR, Baltimore, MD 21201 USA

[3] Univ Maryland, Ctr Adv Chron Pain Res, Baltimore, MD 21201 USA

关键词: Chronic traumatic brain injury;    Neurodegeneration;    Neuroinflammation;    Cognition;    Depression;    Microglia;   

DOI  :  10.1016/j.nbd.2019.104713

来源: Elsevier

【 摘 要 】

Traumatic brain injury (TBI) can cause progressive neurodegeneration, sustained neuroinflammation and chronic neurological dysfunction. Few experimental studies have explored the long-term neurobehavioral and functional cellular changes beyond several months. The present study examined the effects of a single moderate-level TBI on functional outcome 8 months after injury. Male C57BL/6 mice were subjected to controlled cortical impact injury and followed for changes in motor performance, learning and memory, as well as depressive-like and social behavior. We also used a novel flow cytometry approach to assess cellular functions in freshly isolated neurons and microglia from the injured tissue. There were marked and diverse, sustained neurobehavioral changes in injured mice. Compared to sham controls, chronic TBI mice showed long-term deficits in gait dynamics, nest building, spatial working memory and recognition memory. The tail suspension, forced swim, and sucrose consumption tests showed a marked depressive-like phenotype that was associated with impaired sociability. At the cellular level, there were lower numbers of Thy1 (+) Tuj1 (+) neurons and higher numbers of activated CD45(lo)CD11b (+) microglia. Functionally, both neurons and microglia exhibited significantly higher levels of oxidative stress after injury. Microglia exhibited chronic deficits in phagocytosis of E. coin bacteria, and increased uptake of myelin and dying neurons. Living neurons showed decreased expression of synaptophysin and postsynaptic density (PSD)-95, along with greater numbers of microtubule-associated protein light chain 3 (LC3)-positive autophagosomes and increased mitochondrial mass that suggest dysregulation of autophagy. In summary, the late neurobehavioral changes found after murine TBI are similar to those found chronically after moderate-severe human head injury. Importantly, such changes are associated with microglial dysfunction and changes in neuronal activity.