Synthesis of fluorescent nucleoside analogs as probes for 2′-deoxyribonucleoside kinases

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:20

Article

Li, Yongfeng¹  Soni, Priti B.¹  Liu, Lingfeng¹  Zhang, Xiao¹  Liotta, Dennis C.¹  Lutz, Stefan¹ 

[1] Emory Univ, Dept Chem, Atlanta, GA 30322 USA

关键词: Nucleoside kinase;    Fluorescent reporter;    Nucleoside analog;   

DOI  :  10.1016/j.bmcl.2009.12.097

来源: Elsevier

【 摘 要 】

We are reporting on the synthesis of fluorescent nucleoside analogs with modified sugar moieties (e.g., sugars other than ribose and 2'-deoxyribose). Four novel derivatives of the fluorescent thymidine analog 6-methyl-3-(beta-D-2'-deoxyribofuranosyl) furano-[2,3-d]pyrimidin-2-one were synthesized via Sonogashira reaction and subsequent copper-catalyzed cycloaddition. These compounds represent promising tools for studying nucleoside metabolism inside living cells, as well as for screening directed evolution libraries of 2'-deoxyribonucleoside kinases with new and improved activity for the corresponding nucleoside analogs. (C) 2009 Elsevier Ltd. All rights reserved.

【 授权许可】

Free