Differential incorporation of 1‐β‐D‐arabinofuranosylcytosine and 9‐β‐D‐arabinofuranosylguanine into nuclear and mitochondrial DNA

FEBS Letters

Karlsson, Anna¹  Zhu, Chaoyong¹  Johansson, Magnus¹ 

[1] Division of Clinical Virology, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden

关键词: Nucleoside analog;    Nucleoside kinase;    1-β-D-Arabinofuranosylcytosine;    9-β-D-Arabinofuranosylguanine;    Mitochondrial DNA synthesis;    dCK;    deoxycytidine kinase;    dGK;    deoxyguanosine kinase;    araC;    1-β-D-arabinofuranosylcytosine;    araG;    9-β-D-arabinofuranosylguanine;    CHO;    Chinese hamster ovary;   

DOI  :  10.1016/S0014-5793(00)01569-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The anti-leukemic nucleoside analogs 1-β-D-arabinofuranosylcytosine (araC) and 9-β-D-arabinofuranosylguanine (araG) are dependent on intracellular phosphorylation for pharmacological activity. AraC is efficiently phosphorylated by deoxycytidine kinase (dCK). Although araG is phosphorylated by dCK in vitro, it is a preferred substrate of mitochondrial deoxyguanosine kinase. We have used autoradiography to show that araC was incorporated into nuclear DNA in Molt-4 and CEM T-lymphoblastoid cells as well as in Chinese hamster ovary cells. In contrast, araG was predominantly incorporated into mitochondrial DNA in the investigated cell lines, without detectable incorporation into nuclear DNA. These data suggest that the molecular targets of araG and araC may differ.

【 授权许可】

Unknown