Cloning of mouse mitochondrial thymidine kinase 2 cDNA

FEBS Letters

Wettin, Katarina¹  Zheng, Xinyu¹  Karlsson, Anna¹  Zhu, Chaoyong¹  Johansson, Magnus¹ 

[1] Division of Clinical Virology, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden

关键词: Nucleoside analog;    Nucleoside kinase;    Mitochondrial toxicity;    Anti-viral therapy;    Mitochondrial import signal;    TK1;    thymidine kinase 1;    TK2;    thymidine kinase 2;    GFP;    green fluorescent protein;    EST;    expressed sequence tag;   

DOI  :  10.1016/S0014-5793(99)01325-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of anti-viral nucleoside analogs by mitochondrial thymidine kinase 2 (TK2) has been implicated as a mechanism for the mitochondrial toxicity caused by several of these compounds. We have cloned the cDNA of mouse TK2 and shown that the enzyme is targeted to the mitochondria when expressed in fusion with the green fluorescent protein. This is the first report on the cloning of a mitochondrial TK2 and will contribute to elucidate the role of TK2 in the pharmacological activation of nucleoside analogs.

【 授权许可】

Unknown