Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes 1

FEBS Letters

Karlsson, Anna¹  Norda, Ameli¹  Johansson, Magnus¹ 

[1] Division of Clinical Virology, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden

关键词: Nucleoside analog;    Nucleoside kinase;    Transcription regulation;    Nucleoside metabolism;    dCK;    deoxycytidine kinase;   

DOI  :  10.1016/S0014-5793(00)02347-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deoxycytidine kinase (dCK) phosphorylates several anti-cancer and anti-viral nucleoside analogs. The enzyme is predominantly expressed in lymphoid tissues regulated by an unknown mechanism. We have cloned and sequenced the 20 kbp mouse dCK gene and ≈1.7 kbp of the 5′ flanking regions of both the human and mouse dCK genes. Five major inter-species conserved motifs were identified in the 5′ region including the transcription initiation region, an SP1 site and two closely located putative octamer transcription factor sites. Luciferase reporter experiments showed that the human dCK 5′ region efficiently initiated transcription but no tissue regulatory element could be identified.

【 授权许可】

Unknown