Expression of a dynamin 2 mutant associated with Charcot-Marie-Tooth disease leads to aberrant actin dynamics and lamellipodia formation

NEUROSCIENCE LETTERS

卷:628

Article

Yamada, Hiroshi^1,2  Kobayashi, Kinue^1,3  Zhang, Yubai¹  Takeda, Tetsuya^1,2  Takei, Kohji^1,2 

[1] Okayama Univ, Dept Neurosci, Grad Sch Med Dent & Pharmaceut Sci, Kita Ku, 2-5-1 Shikata Cho, Okayama 7008558, Japan

[2] Japan Sci & Technol Agcy, CREST, Kita Ku, 2-5-1 Shikata Cho, Okayama 7008558, Japan

[3] Tohoku Univ, Inst Dev Aging & Canc, Dept Mol Oncol, Aoba Ku, 4-1 Seiryou Machi, Sendai, Miyagi 9808575, Japan

关键词: Charcot-Marie-Tooth disease;    Dynamin;    Actin;    Stress fiber;    Endocytosis;   

DOI  :  10.1016/j.neulet.2016.06.030

来源: Elsevier

【 摘 要 】

Specific mutations in dynamin 2 are linked to Charcot-Marie-Tooth disease (CMT), an inherited peripheral neuropathy. However, the effects of these mutations on dynamin function, particularly in relation to the regulation of the actin cytoskeleton remain unclear. Here, selected CMT-associated dynamin mutants were expressed to examine their role in the pathogenesis of CMT in U2OS cells. Ectopic expression of the dynamin CMT mutants 555 Delta 3 and K562E caused an approximately 50% decrease in serum stimulation dependent lamellipodia formation; however, only K562E caused aberrations in the actin cytoskeleton. Immunofluorescence analysis showed that the K562E mutation resulted in the disappearance of radially aligned actin bundles and the simultaneous appearance of F-actin clusters. Live-cell imaging analyses showed F-actin polymers of decreased length assembled into immobile clusters in K562E-expressing cells. The K562E dynamin mutant colocalized with the F-actin clusters, whereas its colocalization with clathrin-coated pit marker proteins was decreased. Essentially the same results were obtained using another cell line, HeLa and NG108-15 cells. The present study is the first to show the association of dynamin CMT mutations with aberrant actin dynamics and lamellipodia, which may contribute to defective endocytosis and myelination in Schwann cells in CMT. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.

【 授权许可】

Free