Newly identified type of β actin reduces invasiveness of mouse B16‐melanoma

FEBS Letters

Taniguchi, Shun'ichiro¹  Baba, Tsuneo¹  Sadano, Hiroyuki¹ 

[1] Department of Experimental Cell Research, Medical Institute for Bioregulation, Kyushu University 69, Maidashi 3-1-1, Higashi-ku, Fukuoka 812, Japan

关键词: Actin;    DNA transfer;    Stress fiber;    Cytoskeleton;   

DOI  :  10.1016/0014-5793(90)80363-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low metastatic parent B16 melanoma and isolated B16-F1 cell lines have a third actin designated as βm(A^x:previously). ßm actin is scantily or not at all detected in highly metastatic cell lines, such as B16-F10 and BL6. To directly assess the physiological role of ßm in phenotypic changes of B16 melanoma, we transfected expression plasmids of βm into B16-F10 cells. The actin expressed in the transfectants is located largely in cytoskeletal fractios. The transfectants exhibited a larger number of stress fibers and a lower invasiveness than did the recipient cells. Thus, βm actin plays an important role in the organization of actin stress fibers, the result being a decrease in invasiveness of B16 melanoma.

【 授权许可】

Unknown