Microinjection of catalytic subunit of cyclic AMP‐dependent protein kinases triggers acute morphological changes in thyroid epithelial cells

FEBS Letters

Rickaert, Fabienne³  Authelet, Michèle²  Hofmann, Franz¹  Roger, Pierre P.³  Dumont, Jacques E.³  Huez, Georges² 

[1] Laboratoire d'Anatomie Pathologique et de Microscopie Electronique, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium;Laboratoire de Chimie Biologique, 67 rue des Chevaux, B-1640 Rhode St Genèse, Free University of Brussels, Belgium and Physiologische Chemie, Universität des Saarlandes, D-6650 Homburg-Saar, FRG;Institut de Recherche Interdisciplinaire, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium

关键词: cyclic AMP-dependent protein kinase;    Microinjection;    Actin;    Stress fiber;    Thyroid cell;   

DOI  :  10.1016/0014-5793(88)80781-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In dog thyroid epithelial cells in primary culture, thyrotropin acting through cyclic AMP induced rapid morphological changes associated with complete disruption of actin containing stress fibers. This modification preceded cell retraction and rounding up. These morphological effects were also induced by glass capillary microinjection of purified catalytic subunit of cAMP-dependent protein kinase. This provides the first direct evidence in intact cells that catalytic subunit, which is released upon activation of cAMP-dependent protein kinases, is responsible for cAMP-dependent morphological transformation.

【 授权许可】

Unknown