Evidence that acute taurine treatment alters extracellular AMP hydrolysis and adenosine deaminase activity in zebrafish brain membranes

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Article

Rosemberg, Denis Broock^1,2  Kist, Luiza Wilges³  Etchart, Renata Jardim⁴  Rico, Eduardo Pacheco^1,2  Langoni, Andrei Silveira⁴  Dias, Renato Dutra⁴  Bogo, Mauricio Reis^3,5  Bonan, Carla Denise^4,5  Souza, Diogo Onofre^1,2 

[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, Programa Posgrad Bioquim, BR-90035003 Porto Alegre, RS, Brazil

[2] INCT EN, BR-90035003 Porto Alegre, RS, Brazil

[3] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Biol Celular & Mol, Lab Biol Genom & Mol, BR-90619900 Porto Alegre, RS, Brazil

[4] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Biol Celular & Mol, Lab Neuroquim & Psicofarmacol, BR-90619900 Porto Alegre, RS, Brazil

[5] INCT TM, BR-90035003 Porto Alegre, RS, Brazil

关键词: Taurine;    Zebrafish;    Ectonucleotidases;    Adenosine deaminase;    Brain;   

DOI  :  10.1016/j.neulet.2010.06.062

来源: Elsevier

【 摘 要 】

Taurine is one of the most abundant free amino acids in excitable tissues. In the brain, extracellular taurine may act as an inhibitory neurotransmitter, neuromodulator, and neuroprotector. Nucleotides are ubiquitous signaling molecules that play crucial roles for brain function. The inactivation of nucleotide-mediated signaling is controlled by ectonucleotidases, which include the nucleoside triphosphate diphosphohydrolase (NTPDase) family and ecte-5'-nucleotidase. These enzymes hydrolyze ATP/GTP to adenosine/guanosine, which exert a modulatory role controlling several neurotransmitter systems. The nucleoside adenosine can be inactivated in extracellular or intracellular milieu by adenosine deaminase (ADA). In this report, we tested whether acute taurine treatment at supra-physiological concentrations alters NTPDase, ecto-5'-nucleotidase, and ADA activities in zebrafish brain. Fish were treated with 42, 150, and 400 mg L-1 taurine for 1 h, the brains were dissected and the enzyme assays were performed. Although the NTPDase activities were not altered, 150 and 400 mg L-1 taurine increased AMP hydrolysis (128 and 153%, respectively) in zebrafish brain membranes and significantly decreased ecto-ADA activity (29 and 38%, respectively). In vitro assays demonstrated that taurine did not change AMP hydrolysis, whereas it promoted a significant decrease in ecto-ADA activity at 150 and 400 mg L-1 (24 and 26%, respectively). Altogether, our data provide the first evidence that taurine exposure modulates the ecto-enzymes responsible for controlling extracellular adenosine levels in zebrafish brain. These findings could be relevant to evaluate potential beneficial effects promoted by acute taurine treatment in the central nervous system (CNS) of this species. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

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