Acute administration of cocaine reduces metabotropic glutamate receptor 8 protein expression in the rat striatum in vivo

NEUROSCIENCE LETTERS

卷:449

Article

Zhang, Guo-Chi¹  Vu, Khang²  Parelkar, Nikhil K.³  Mao, Li-Min¹  Stanford, Ian M.¹  Fibuch, Eugene E.²  Wang, John Q.^1,2,3 

[1] Univ Missouri, Sch Med, Dept Basic Med Sci, Kansas City, MO 64108 USA

[2] Univ Missouri, Sch Med, Dept Anesthesiol, Kansas City, MO 64108 USA

[3] Univ Missouri, Sch Med, Dept Pharmacol, Kansas City, MO 64108 USA

关键词: Dopamine;    Cortex;    Hippocampus;    Cauclate;    Nucleus accumbens;    Addiction;    Reward;   

DOI  :  10.1016/j.neulet.2008.11.008

来源: Elsevier

【 摘 要 】

Metabotropic glutamate receptors (mGluRs) are densely expressed in the limbic system of the mammalian brain. Increasing evidence suggests a critical role of mGluRs in the pathogenesis of various mental illnesses, including drug abuse and addiction. In this study, we investigated the effect of psychostimulant, cocaine, on protein expression of a specific mGluR subtype, mGluR8, in the rat forebrain in vivo. A rabbit antibody against the extracellular N-terminus of mGluR8 was developed to detect changes in mGluR8 proteins in immunoblot assays. With this antibody, we found that acute systemic injection of cocaine reduced mGluR8 protein levels in the striatum. The reduction of mGluR8 proteins was rapid and transient as it was induced 25 min after cocaine injection and returned to the normal level by 6 h. No significant change in mGluR8 protein levels in the prefrontal cortex and the hippocampus was observed following cocaine administration. These data demonstrate that protein expression of mGluR8 is subject to the modulation by dopamine stimulation. Acute exposure to cocaine results in a dynamic and region-specific downregulation of mGluR8 expression in the striatum. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free