The Human Explanted Heart Program: A translational bridge for cardiovascular medicine

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1867

Review

Zhang, Hao^1,2  Viveiros, Anissa^2,3  Nikhanj, Anish^1,2  Quynh Nguyen^1,2  Wang, Kaiming^1,2  Wang, Wei^2,4  Freed, Darren H.^2,4  Mullen, John C.^2,4  MacArthur, Roderick^2,4  Kim, Daniel H.^1,2  Tymchak, Wayne^1,2  Sergi, Consolato M.^2,5  Kassiri, Zamaneh^2,3  Wang, Shaohua^2,4  Oudit, Gavin Y.^1,2 

[1] Univ Alberta, Fac Med & Dent, Dept Med, Div Cardiol, Edmonton, AB, Canada

[2] Univ Alberta, Mazankowski Alberta Heart Inst, Edmonton, AB, Canada

[3] Univ Alberta, Fac Med & Dent, Dept Physiol, Edmonton, AB, Canada

[4] Univ Alberta, Fac Med & Dent, Dept Surg, Div Cardiac Surg, Edmonton, AB, Canada

[5] Univ Alberta, Fac Med & Dent, Dept Lab Med & Pathol, Div Anat Pathol, Edmonton, AB, Canada

关键词: Tissue biobank;    Human explanted heart;    Heart failure;    Pediatric heart diseases;    Ventricular assist device;    Translational research;   

DOI  :  10.1016/j.bbadis.2020.165995

来源: Elsevier

【 摘 要 】

The progression of cardiovascular research is often impeded by the lack of reliable disease models that fully recapitulate the pathogenesis in humans. These limitations apply to both in vitro models such as cell-based cultures and in vivo animal models which invariably are limited to simulate the complexity of cardiovascular disease in humans. Implementing human heart tissue in cardiovascular research complements our research strategy using preclinical models. We established the Human Explanted Heart Program (HELP) which integrates clinical, tissue and molecular phenotyping thereby providing a comprehensive evaluation into human heart disease. Our collection and storage of biospecimens allow them to retain key pathogenic findings while providing novel insights into human heart failure. The use of human non-failing control explanted hearts provides a valuable comparison group for the diseased explanted hearts. Using HELP we have been able to create a tissue repository which have been used for genetic, molecular, cellular, and histological studies. This review describes the process of collection and use of explanted human heart specimens encompassing a spectrum of pediatric and adult heart diseases, while highlighting the role of these invaluable specimens in translational research. Furthermore, we highlight the efficient procurement and bio-preservation approaches ensuring analytical quality of heart specimens acquired in the context of heart donation and transplantation.

【 授权许可】

Free