The effect of red light and far-red light conditions on secondary metabolism in Agarwood

BMC Plant Biology

Research Article

Chien-Yu Chen¹  Hsiao-Feng Lo²  I-Hsuan Lu³  Shu-Hwa Chen³  Chung-Yen Lin⁴  Shih-Tong Jeng⁵  Mei-Ju Chu⁶  Li-Chun Huang⁶  Long-Fang O. Chen⁶  Tony Chien-Yen Kuo⁷  Chuan-Hung Chen⁸ 

[1] Department of Bio-industrial Mechatronics Engineering, National Taiwan University, 106, Taipei, Taiwan;Center for Systems Biology, National Taiwan University, 106, Taipei, Taiwan;Department of Horticulture and Landscape Architecture, National Taiwan University, 106, Taipei, Taiwan;Institute of Information Science, Academia Sinica, 115, Taipei, Taiwan;Institute of Information Science, Academia Sinica, 115, Taipei, Taiwan;Division of Biostatistics and Bioinformatics, Institute of Population Health Sciences, National Health Research Institutes, 350, Zhunan, Taiwan;Institute of Fisheries Science, College of Life Science, National Taiwan University, 106, Taipei, Taiwan;Institute of Plant Biology, College of Life Science, National Taiwan University, 106, Taipei, Taiwan;Institute of Plant and Microbial Biology, Academia Sinica, 128 Sec. 2, Academia Rd, 11529, Nankang, Taipei, Taiwan;Institute of Plant and Microbial Biology, Academia Sinica, 128 Sec. 2, Academia Rd, 11529, Nankang, Taipei, Taiwan;Department of Bio-industrial Mechatronics Engineering, National Taiwan University, 106, Taipei, Taiwan;Institute of Plant and Microbial Biology, Academia Sinica, 128 Sec. 2, Academia Rd, 11529, Nankang, Taipei, Taiwan;Institute of Plant Biology, College of Life Science, National Taiwan University, 106, Taipei, Taiwan;

关键词: Agarwood;    Aquilaria agallocha;    Genome;    Secondary metabolism;    Red light;    Cucurbitacin;   

DOI  :  10.1186/s12870-015-0537-y

 received in 2015-02-01, accepted in 2015-03-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAgarwood, a heartwood derived from Aquilaria trees, is a valuable commodity that has seen prevalent use among many cultures. In particular, it is widely used in herbal medicine and many compounds in agarwood are known to exhibit medicinal properties. Although there exists much research into medicinal herbs and extraction of high value compounds, few have focused on increasing the quantity of target compounds through stimulation of its related pathways in this species.ResultsIn this study, we observed that cucurbitacin yield can be increased through the use of different light conditions to stimulate related pathways and conducted three types of high-throughput sequencing experiments in order to study the effect of light conditions on secondary metabolism in agarwood. We constructed genome-wide profiles of RNA expression, small RNA, and DNA methylation under red light and far-red light conditions. With these profiles, we identified a set of small RNA which potentially regulates gene expression via the RNA-directed DNA methylation pathway.ConclusionsWe demonstrate that light conditions can be used to stimulate pathways related to secondary metabolism, increasing the yield of cucurbitacins. The genome-wide expression and methylation profiles from our study provide insight into the effect of light on gene expression for secondary metabolism in agarwood and provide compelling new candidates towards the study of functional secondary metabolic components.

【 授权许可】

Unknown   
© Kou et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.