Vivax malaria in Mauritania includes infection of a Duffy-negative individual

Malaria Journal

Research

Ali Ould Mohamed Salem Boukhary¹  Mohamed Salem Ould Ahmedou Salem¹  Khadijetou Mint Lekweiry²  Jamal Eddine Hafid³  Hervé Bogreau⁴  Nathalie Wurtz⁴  Bruno Pradines⁴  Sébastien Briolant⁴  Leonardo K Basco⁴  Christophe Rogier⁵  Jean-François Trape⁶ 

[1] Laboratoire de Biotechnologies, Faculté des Sciences et Techniques, Université de Nouakchott, Mauritania;Laboratoire de Biotechnologies, Faculté des Sciences et Techniques, Université de Nouakchott, Mauritania;UFR Biologie et Santé, Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, Marrakech, Morocco;Laboratoire Aliments, Environnement et Santé (LAES), Faculté des Sciences et Techniques, Université Cadi Ayyad, Marrakech, Morocco;UFR Biologie et Santé, Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, Marrakech, Morocco;Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Recherche Biomédicale des Armées, Allée du médecin colonel Eugène Jamot, Parc du Pharo, BP 60109, 13262, Marseille Cedex 07, France;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236, Marseille, France;Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Recherche Biomédicale des Armées, Allée du médecin colonel Eugène Jamot, Parc du Pharo, BP 60109, 13262, Marseille Cedex 07, France;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236, Marseille, France;Institut Pasteur de Madagascar, Antananarivo, Madagascar;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236, Marseille, France;Laboratoire de Paludologie, Institut de Recherche pour le Développement, Dakar, Sénégal;

关键词: Plasmodium vivax;    Duffy blood group;    Mauritania;    polymorphism;    malaria;   

DOI  :  10.1186/1475-2875-10-336

 received in 2011-09-19, accepted in 2011-11-03,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundDuffy blood group polymorphisms are important in areas where Plasmodium vivax is present because this surface antigen is thought to act as a key receptor for this parasite. In the present study, Duffy blood group genotyping was performed in febrile uninfected and P. vivax-infected patients living in the city of Nouakchott, Mauritania.MethodsPlasmodium vivax was identified by real-time PCR. The Duffy blood group genotypes were determined by standard PCR followed by sequencing of the promoter region and exon 2 of the Duffy gene in 277 febrile individuals. Fisher's exact test was performed in order to assess the significance of variables.ResultsIn the Moorish population, a high frequency of the FYBES/FYBES genotype was observed in uninfected individuals (27.8%), whereas no P. vivax-infected patient had this genotype. This was followed by a high level of FYA/FYB, FYB/FYB, FYB/FYBES and FYA/FYBES genotype frequencies, both in the P. vivax-infected and uninfected patients. In other ethnic groups (Poular, Soninke, Wolof), only the FYBES/FYBES genotype was found in uninfected patients, whereas the FYA/FYBES genotype was observed in two P. vivax-infected patients. In addition, one patient belonging to the Wolof ethnic group presented the FYBES/FYBES genotype and was infected by P. vivax.ConclusionsThis study presents the Duffy blood group polymorphisms in Nouakchott City and demonstrates that in Mauritania, P. vivax is able to infect Duffy-negative patients. Further studies are necessary to identify the process that enables this Duffy-independent P. vivax invasion of human red blood cells.

【 授权许可】

CC BY   
© Wurtz et al; licensee BioMed Central Ltd. 2011