Transcriptome profiling analysis reveals metabolic changes across various growth phases in Bacillus pumilus BA06

BMC Microbiology

Research Article

Yong-Cheng Liu¹  Gang Liu¹  Xue-Mei Tan¹  Xiao-Jie Cheng¹  Lin-Li Han¹  Huan-Huan Shao¹  Chao-Ying Xie¹  Hong Feng¹  Hai-Yan Wang¹ 

[1] Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Sichuan University, 610064, Chengdu, Sichuan, People’s Republic of China;College of Life Sciences, Sichuan University, 610064, Chengdu, Sichuan, People’s Republic of China;

关键词: Bacillus pumilus;    Bacterial flagella;    Chemotaxis;    Gene expression;    Gene regulation;    Protease;    RNA-seq;    Sporulation;    Stress response;    Transcriptome;    Tricarboxylic acid cycle;   

DOI  :  10.1186/s12866-017-1066-7

 received in 2017-03-01, accepted in 2017-07-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes. It is necessary to understand the metabolic processes during cellular growth. Here, an RNA-seq based transcriptome analysis was applied to examine B. pumilus BA06 across various growth stages to reveal metabolic changes under two conditions.ResultsBased on the gene expression levels, changes to metabolism pathways that were specific to various growth phases were enriched by KEGG analysis. Upon entry into the transition from the exponential growth phase, striking changes were revealed that included down-regulation of the tricarboxylic acid cycle, oxidative phosphorylation, flagellar assembly, and chemotaxis signaling. In contrast, the expression of stress-responding genes was induced when entering the transition phase, suggesting that the cell may suffer from stress during this growth stage. As expected, up-regulation of sporulation-related genes was continuous during the stationary growth phase, which was consistent with the observed sporulation. However, the expression pattern of the various extracellular proteases was different, suggesting that the regulatory mechanism may be distinct for various proteases. In addition, two protein secretion pathways were enriched with genes responsive to the observed protein secretion in B. pumilus. However, the expression of some genes that encode sporulation-related proteins and extracellular proteases was delayed by the addition of gelatin to the minimal medium.ConclusionsThe transcriptome data depict global alterations in the genome-wide transcriptome across the various growth phases, which will enable an understanding of the physiology and phenotype of B. pumilus through gene expression.

【 授权许可】

CC BY   
© The Author(s). 2017