Effects of exercise on brain and peripheral inflammatory biomarkers induced by total sleep deprivation in rats

Journal of Inflammation

Research

H. Geoffroy¹  T. Gallopin¹  S. Ciret²  C. Langrume²  G. Dispersyn³  C. Drogou³  D. Gomez-Merino³  F. Sauvet³  M. Chennaoui⁴ 

[1] Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005, Paris, France;Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005, Paris, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France;Armed Forces Biomedical Research Institute (IRBA), B.P.73, 91223, Brétigny-sur-Orge, Cedex, France;

关键词: Cytokines;    Hormones;    Brain;    Periphery;    Sleep deprivation;    Exercise;   

DOI  :  10.1186/s12950-015-0102-3

 received in 2015-06-25, accepted in 2015-09-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPhysical exercise induces neuroprotection through anti-inflammatory effects and total sleep deprivation is reported an inflammatory process. We examined whether 7 weeks of exercise training attenuates markers of inflammation during total sleep deprivation (24-h wakefulness) in the rat brain and periphery.MethodsFour groups of 10 rats were investigated: Sedentary control, Sedentary sleep-deprived, Exercised control, and Exercised sleep-deprived. Sleep deprivation and exercise training were induced using slowly rotating wheels and a motorized treadmill. We examined mRNA expression of pro-inflammatory (IL-1β, TNF-α, and IL-6) cytokine-related genes using real-time PCR, and protein levels in the hippocampus and frontal cortex, as well as circulating concentrations.ResultsCompared to Sedentary control rats, hippocampal and cortical IL-1β mRNA expressions in Sedentary sleep-deprived rats were up-regulated (p < 0.05 and p < 0.01 respectively). At the protein level, hippocampal IL-1β and TNF-α and cortical IL-6 contents were higher in Sedentary sleep-deprived rats (p < 0.001, p < 0.05, p < 0.05, respectively). Peripherally, TNF-α, IL-6 and norepinephrine concentrations were higher in Sedentary sleep-deprived rats compared to Sedentary control (p < 0.01, p < 0.001, p < 0.01, respectively). Exercise training reduced the sleep deprivation-induced hippocampal IL-1β increases (mRNA expression and protein content) (p < 0.05 and p < 0.001), and TNF-α content (p < 0.001). At the periphery, exercise reduced sleep deprivation-induced increase of IL-6 concentration (p < 0.05) without effect on TNF-α and norepinephrine.ConclusionsWe demonstrate that a 7-week exercise training program before acute total sleep deprivation prevents pro-inflammatory responses in the rat hippocampus, particularly the IL-1β cytokine at the gene expression level and protein content.

【 授权许可】

CC BY   
© Chennaoui et al. 2015