Journal of Inflammation | |
Effects of exercise on brain and peripheral inflammatory biomarkers induced by total sleep deprivation in rats | |
Research | |
H. Geoffroy1  T. Gallopin1  S. Ciret2  C. Langrume2  G. Dispersyn3  C. Drogou3  D. Gomez-Merino3  F. Sauvet3  M. Chennaoui4  | |
[1] Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005, Paris, France;Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005, Paris, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France;Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France;Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France;Armed Forces Biomedical Research Institute (IRBA), B.P.73, 91223, Brétigny-sur-Orge, Cedex, France; | |
关键词: Cytokines; Hormones; Brain; Periphery; Sleep deprivation; Exercise; | |
DOI : 10.1186/s12950-015-0102-3 | |
received in 2015-06-25, accepted in 2015-09-23, 发布年份 2015 | |
来源: Springer | |
【 摘 要 】
BackgroundPhysical exercise induces neuroprotection through anti-inflammatory effects and total sleep deprivation is reported an inflammatory process. We examined whether 7 weeks of exercise training attenuates markers of inflammation during total sleep deprivation (24-h wakefulness) in the rat brain and periphery.MethodsFour groups of 10 rats were investigated: Sedentary control, Sedentary sleep-deprived, Exercised control, and Exercised sleep-deprived. Sleep deprivation and exercise training were induced using slowly rotating wheels and a motorized treadmill. We examined mRNA expression of pro-inflammatory (IL-1β, TNF-α, and IL-6) cytokine-related genes using real-time PCR, and protein levels in the hippocampus and frontal cortex, as well as circulating concentrations.ResultsCompared to Sedentary control rats, hippocampal and cortical IL-1β mRNA expressions in Sedentary sleep-deprived rats were up-regulated (p < 0.05 and p < 0.01 respectively). At the protein level, hippocampal IL-1β and TNF-α and cortical IL-6 contents were higher in Sedentary sleep-deprived rats (p < 0.001, p < 0.05, p < 0.05, respectively). Peripherally, TNF-α, IL-6 and norepinephrine concentrations were higher in Sedentary sleep-deprived rats compared to Sedentary control (p < 0.01, p < 0.001, p < 0.01, respectively). Exercise training reduced the sleep deprivation-induced hippocampal IL-1β increases (mRNA expression and protein content) (p < 0.05 and p < 0.001), and TNF-α content (p < 0.001). At the periphery, exercise reduced sleep deprivation-induced increase of IL-6 concentration (p < 0.05) without effect on TNF-α and norepinephrine.ConclusionsWe demonstrate that a 7-week exercise training program before acute total sleep deprivation prevents pro-inflammatory responses in the rat hippocampus, particularly the IL-1β cytokine at the gene expression level and protein content.
【 授权许可】
CC BY
© Chennaoui et al. 2015
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311109149756ZK.pdf | 1132KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]
- [34]
- [35]
- [36]
- [37]
- [38]
- [39]
- [40]
- [41]
- [42]
- [43]
- [44]
- [45]
- [46]
- [47]
- [48]