Genetic diversity of Plasmodium falciparum isolates from Baka Pygmies and their Bantu neighbours in the north of Gabon

Malaria Journal

Research

Nancy Diamella-Moukodoum¹  Lucrèce M. Délicat-Loembet¹  Larson Boundenga²  Bertrand Mvé-Ondo³  Benjamin Ollomo⁴  Lauriane Yacka Mouele⁴  Virginie Rougeron⁵  Franck Prugnolle⁵  Dieudonné Nkoghe⁶  Simone Lemmers⁷  Patrick Durand⁸  Eric Elguero⁸  François Renaud⁸  Christophe Paupy⁸  Céline Arnathau⁸  Ulrich Bisvigou⁹ 

[1] Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Département de Biologie Animale, Faculté des Sciences et Techniques, Laboratoire d’Écologie et Biologie évolutive, Université Cheikh AntaDiop de Dakar, BP 5005, Dakar, Senegal;Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Ecole Doctorale Régionale d’Afrique Centrale en Infectiologie Tropicale, BP 876, Franceville, Gabon;Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Fac de Médecine, UMR-BIOMED, BP 8507, Libreville, Gabon;Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle (MIVEGEC), UMR CNRS 5290/IRD 224, Université Montpellier, CHRU de Montpellier, 39 Avenue Charles Flahault, 34295, Montpellier, France;Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon;Ministry of Health, BP 50, Libreville, Gabon;Department of Anthropology, Durham University, Dawson Building, South Road, DH1 3LE, Durham, England, UK;Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle (MIVEGEC), UMR CNRS 5290/IRD 224, Université Montpellier, CHRU de Montpellier, 39 Avenue Charles Flahault, 34295, Montpellier, France;Ministry of Health, BP 50, Libreville, Gabon;

关键词: Malaria;    Genetic diversity;    Population structure;    Microsatellite loci;    Antigenic determinants;    Baka Pygmies;    Bantus;   

DOI  :  10.1186/s12936-015-0862-5

 received in 2015-06-06, accepted in 2015-08-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThere have been many reports on the population genetic structure of Plasmodium falciparum from different endemic regions especially sub-Saharan Africa. However, few studies have been performed on neglected populations, such as the Pygmy populations. In this study, the population genetic structure of P. falciparum was investigated in the Baka Pygmies of Gabon and compared to that observed in neighboring villages composed mostly of Bantu farmers.MethodsA total of 342 blood samples were collected from 170 Baka Pygmies and 172 Bantus in the north of Gabon (Woleu Ntem Province). Plasmodium infections were characterized by sequencing a portion of the parasite cytochrome b gene. Population genetic structure of P. falciparum in the different villages was analysed using microsatellite markers and genes coding for antigenic proteins (MSP1, MSP2, GLURP, and EBA-175).ResultsOverall, prevalence of P. falciparum was around 57 % and no significant difference of prevalence was observed between Pygmies and Bantus. No significant differences of population genetic structure of P. falciparum was found between Pygmy and Bantu people except for one antigen-coding gene, glurp, for which genetic data suggested the existence of a potentially disruptive selection acting on this gene in the two types of populations. The genetic structure of P. falciparum followed a pattern of isolation by distance at the scale of the study.ConclusionThe prevalence and genetic diversity of P. falciparum observed in Baka demonstrates a significant transmission of the parasite in this population, and some exchanges of parasites with Bantu neighbours. Despite that, some antigen-coding genes seem to have had a particular evolutionary trajectory in certain Pygmy populations due to specific local human and/or mosquito characteristics.

【 授权许可】

CC BY   
© Mvé-Ondo et al. 2015