The genetic polymorphism of merozoite surface protein-1 in Plasmodium falciparum isolates from Aceh province, Indonesia

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Jamil, K.F.^1 ; Supargiyono, S.^2 ; Syafruddin, D.^3 ; Pratama, N.^3 ; Silvy, S.^3

Division of Tropical and Infectious Diseases, Department of Internal Medicine, Medical Faculty of Syiah Kuala University, Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia^1

Center for Tropical Medicine Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia^2

Eijkman Institute for Molecular Biology, Jakarta, Indonesia^3

关键词: Genetic diversity;    Genetic polymorphisms;    Laboratory test;    Merozoite surface proteins;    Mixed infections;    P. falciparum;    Physical examinations;    Plasmodium falciparum;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012037/pdf
DOI  :  10.1088/1755-1315/125/1/012037

来源: IOP

【 摘 要 】

An estimated of 3.3 million Indonesian population were infected with malaria. However, extensive genetic polymorphism of the field isolates msp-1 of P. falciparum represents a major obstacle for the development of malaria treatment. The aim of this study was to investigate the genetic diversity of msp-1 genotype in field isolates of P. falciparum collected in Aceh Province. A total of 90 patients with malaria (+) were selected from eleven district hospitals in Aceh from 2013-2015. Data were collected by anamnesis, complete physical examination and laboratory tests for msp-1. All protocols to diagnose malaria followed the WHO 2010 guideline. All samples were stored in Eijkman Biology Molecular Institute, Jakarta. Among 90 samples, 57.7% were male, and 42.3% were female with the most cases found between 21-30 years old. From the allele typing analysis of P. falciparum from Aceh; K1, MAD20, and RO33 allele types were identified. MAD20 type was the highest allele found in this study (57.9%). It was found in single and mixed infection. A moderate level of the mixed allele was also observed.