Microsatellite Analysis on Genetic Variation in Two Populations of Red Mangrove Rhizophora Mangle L. (Rhizophoraceae) and Its Implication to Conservation

1st Annual Applied Science and Engineering Conference

工业技术;自然科学

Basyuni, M.^1 ; Baba, S.^2 ; Oku, H.^3

Department of Forestry, Faculty of Forestry, University of Sumatera Utara, Jl. Tri Dharma Ujung No. 1, Medan, North Sumatera

20155, Indonesia^1

International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^2

Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^3

关键词: Analysis of molecular variances;    Colonizing species;    Genetic structure;    Genetic variation;    Mangrove species;    Micro satellite;    Microsatellite loci;    Rhizophoraceae;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/180/1/012243/pdf
DOI  :  10.1088/1757-899X/180/1/012243

来源: IOP

【 摘 要 】

Two populations of red mangrove Rhizophora mangle L. (Rhizophoraceae) in Ecuador was examined using five microsatellite primers. Microsatellite loci were low polymorphic with 2-5 alleles per locus. The level observed heterozygosity detected overall loci population, ranged from 0.189 to 0.405, indicating that these populations had little or no genetic variation. Furthermore, genetic variation was maintained within population (GST=0.012). Both populations showed high level of inbreeding (FIS=0.695, FST=0.024), suggested that mangrove species behave as colonizing species. The genetic structure was tested by analysis of molecular variance (AMOVA) using the infinite alleles model (F-statistics), found that most variation was among individuals within population (33.3 %) and within individuals in the total populations (66.6 %), whereas there was little variation between populations (0.1 %). An understanding of genetic variation of R. mangle suggested the importance of conservation this species to increase the number of populations.