RAPD markers on genetic diversity in three populations of pisifera type of oil palm (elaeis guineensis)

3rd International Conference on Biological Sciences and Biotechnology

Basyuni, M.^1 ; Prayogi, H.^1 ; Putri, L.A.P.^2 ; Syahputra, I.^3 ; Siregar, E.S.^4 ; Risnasari, I.^1 ; Wati, R.^1 ; Arifiyanto, D.^3

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1, Medan, North Sumatera

20155, Indonesia^1

Department of Agroecotechnology, Faculty of Agriculture, Universitas Sumatera Utara, Medan, North Sumatera

20155, Indonesia^2

Laboratory of Biomolecular of PT Socfin Indonesia, Martebing, Dolok Masihul, Tebing Tinggi, North Sumatra

20991, Indonesia^3

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan

20155, Indonesia^4

关键词: Breeding;    female sterile;    Genetic diversity;    Genetic variation;    Oil palms (Elaeis guineensis);    Polymorphic information contents;    Sumatra;    Sumatra , Indonesia;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012050/pdf
DOI  :  10.1088/1755-1315/130/1/012050

来源: IOP

【 摘 要 】

Palm oil (E. guineensis) is one of the major commodity and contributing largely to non-petroleum oil of Indonesian foreign exchange. E. guineensis has three fruit types, dura (female), pisifera (male), and tenera - a hybrid between dura and pisifera. Pisifera plays an important function in the production of seed oil palm. The purpose of this research is to analyze genetic diversity of pisifera type of E. guineensis from three populations, Yangambi, Lame and Lame further cross in Bangun Bandar, North Sumatra, Indonesia. Eighteen samples for each population were analyzed using six RAPD markers. Results showed that RAPD markers were low polymorphic with 1.49, 1.39, and 1.00 average number alleles detected for Yagambi, Lame, and Lame further cross, respectively. The level of genetic diversity detected for each population was 0.28, 0.22, and 0.21 for Yagambi, Lame, and Lame further cross, respectively, indicating that the populations had little genetic variation. The highest of polymorphic information content (PIC) was found on the P11 primer of Yangambi (0.49) and P10 primer for lame further cross (0.49). By contrast, the lowest PIC belongs to P21 for Lame population (0.01). This data is likely to contributing oil palm breeding.