Assessment of genetic diversity in the endangered populations of Breonadia salicina (Rubiaceae) growing in The Kingdom of Saudi Arabia using inter-simple sequence repeat markers

BMC Genetics

Salim Khan¹  Fahad Al-Qurainy¹  Abdel-Rhman Z Gaafar¹ 

[1] Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

关键词: Conservation biology;    ISSR;    Molecular markers;    Genetic structure;    Genetic variation;    Breonadia salicina;   

Others  :  1085503
DOI  :  10.1186/s12863-014-0109-4

 received in 2013-12-07, accepted in 2014-09-29,  发布年份 2014

【 摘 要 】

Background

Breonadia salicina (Rubiaceae) is a critically endangered plant at the local scale native to southwestern Saudi Arabia. To understand the levels and partitioning of genetic variation across populations and geographical regions of this species, we assessed its genetic diversity using inter-simple sequence repeat (ISSR) markers.

Results

Fourteen ISSR primers selected from 43 primers gave rise to 211 amplified loci, of which 68 were polymorphic. The percentage of polymorphic loci (PPL) at the population level ranged from 17.1 to 23.7%, with an average of 21.3%. Nei’s gene diversity (h) and Shannon’s information index (I) were 0.086 and 0.125, respectively. At the species level, PPL was 32.2%, while h and I were 0.116 and 0.172, respectively. A hierarchical analysis of molecular variance revealed a high level of genetic differentiation among populations (17% of total variance, P = 0.001), consistent with the gene differentiation coefficient (G_ST = 0.256). Nevertheless, the evaluated genetic diversity was very low within populations; while relatively high among populations, levels were insufficient for long-term survival. Saudi Arabian accessions were also compared to accessions of a population from Yemen, where the species is more widespread. The Yemeni population also showed low genetic diversity but clustered separately.

Conclusions

Breonadia salicina in Saudi Arabia is characterized by low within-population genetic diversity and high among-population genetic differentiation. Based on our findings, this locally endangered species is on the verge of local extinction. The species’ survival depends on successful implementation of suggested strategies for its long-term conservation.

【 授权许可】

   
2014 Gaafar et al.; licensee BioMed Central Ltd.

【 图 表 】

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