Genetic diversity of Plasmodium vivax clinical isolates from southern Pakistan using pvcsp and pvmsp1 genetic markers

Malaria Journal

Research

Ali M Thaver¹  Najia K Ghanchi²  Sana Jafri²  Mohammad A Beg²  Afsheen Raza² 

[1] Aga Khan Medical College, PO Box 3500, Stadium Road, 74800, Karachi, Pakistan;Department of Pathology and Microbiology, Aga Khan University, PO Box 3500, Stadium Road, 74800, Karachi, Pakistan;

关键词: Malaria;    Plasmodium vivax;    Pakistan;    Genetic diversity;    Population structure;    Circumsporozoite protein;    Merozoite surface protein 1;   

DOI  :  10.1186/1475-2875-12-16

 received in 2012-09-07, accepted in 2013-01-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPlasmodium vivax is the prevalent malarial species accounting for 70% of malaria burden in Pakistan; however, there is no baseline data on the circulating genotypes. Studies have shown that polymorphic loci of gene encoding antigens pvcsp and pvmsp1 can be used reliably for conducting molecular epidemiological studies. Therefore, this study aimed to bridge the existing knowledge gap on population structure on P. vivax from Pakistan using these two polymorphic genes.MethodsDuring the period January 2008 to May 2009, a total of 250 blood samples were collected from patients tested slide positive for P. vivax, at the Aga Khan University Hospital, Karachi, or its collection units located in Baluchistan and Sindh Province. Nested PCR/RFLP was performed, using pvcsp and pvmsp1 markers to detect the extent of genetic diversity in clinical isolates of P. vivax from southern Pakistan.ResultsA total of 227/250 (91%) isolates were included in the analysis while the remainder were excluded due to negative PCR outcome for P.vivax. Pvcsp analysis showed that both VK 210 (85.5%, 194/227) and VK 247 type (14.5%, 33/227) were found to be circulating in P. vivax isolates from southern Pakistan. A total of sixteen and eighty-seven genotypes of pvcsp and pvmsp-1 were detected respectively.ConclusionThis is the first report from southern Pakistan on characterization of P. vivax isolates confirming that extensively diverse pvcsp and pvmsp1 variants are present within this region. Results from this study provide valuable data on genetic diversity of P. vivax that will be helpful for further epidemiological studies.

【 授权许可】

Unknown   
© Raza et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.