Genetic structure of Plasmodium vivax using the merozoite surface protein 1 icb5-6 fragment reveals new hybrid haplotypes in southern Mexico

Malaria Journal

Research

Ana Wegier¹  José A Nettel²  Lilia González-Cerón²  René Cerritos³ 

[1] CENID-COMEF, Instituto Nacional Investigaciones Forestales, Agrícolas y Pecuarias México, Progreso 5, Barrio Santa Catarina, D.F. 04010, Coyoacán, México;Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Tapachula, Chiapas, México;Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado postal 70-275, 04510, México D.F., México;

关键词: Plasmodium vivax;    Mexico;    Malaria;    Merozoite surface protein 1;    Haplotype network;    Population genetic;    Genetic diversity;    Recombination analysis;   

DOI  :  10.1186/1475-2875-13-35

 received in 2013-07-18, accepted in 2014-01-22,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPlasmodium vivax is a protozoan parasite with an extensive worldwide distribution, being highly prevalent in Asia as well as in Mesoamerica and South America. In southern Mexico, P. vivax transmission has been endemic and recent studies suggest that these parasites have unique biological and genetic features. The msp1 gene has shown high rate of nucleotide substitutions, deletions, insertions, and its mosaic structure reveals frequent events of recombination, maybe between highly divergent parasite isolates.MethodsThe nucleotide sequence variation in the polymorphic icb5-6 fragment of the msp1 gene of Mexican and worldwide isolates was analysed. To understand how genotype diversity arises, disperses and persists in Mexico, the genetic structure and genealogical relationships of local isolates were examined. To identify new sequence hybrids and their evolutionary relationships with other P. vivax isolates circulating worldwide two haplotype networks were constructed questioning that two portions of the icb5-6 have different evolutionary history.ResultsTwelve new msp1 icb5-6 haplotypes of P. vivax from Mexico were identified. These nucleotide sequences show mosaic structure comprising three partially conserved and two variable subfragments and resulted into five different sequence types. The variable subfragment sV1 has undergone recombination events and resulted in hybrid sequences and the haplotype network allocated the Mexican haplotypes to three lineages, corresponding to the Sal I and Belem types, and other more divergent group. In contrast, the network from icb5-6 fragment but not sV1 revealed that the Mexican haplotypes belong to two separate lineages, none of which are closely related to Sal I or Belem sequences.ConclusionsThese results suggest that the new hybrid haplotypes from southern Mexico were the result of at least three different recombination events. These rearrangements likely resulted from the recombination between haplotypes of highly divergent lineages that are frequently distributed in South America and Asia and diversified rapidly.

【 授权许可】

Unknown   
© Cerritos et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.