Unravelling the patterns of host immune responses in Plasmodium vivax malaria and dengue co-infection

Malaria Journal

Research

Maria P G Mourão¹  Marcus V G Lacerda¹  Belisa M L Magalhães¹  Bruno B Andrade²  Vitor R R Mendonça³  Ligia C L Souza³  Manoel Barral-Netto⁴ 

[1] Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil;Universidade do Estado do Amazonas, Manaus, Brazil;Laboratório Integrado de Microbiogia e Imunoregulação (LIMI), Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil;Laboratório Integrado de Microbiogia e Imunoregulação (LIMI), Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil;Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil;Laboratório Integrado de Microbiogia e Imunoregulação (LIMI), Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil;Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil;Instituto de Investigação em Imunologia, Instituto Nacional de Ciência e Tecnologia, São Paulo, Brazil;

关键词: Immune response;    Co-infection;    Dengue;    Plasmodium vivax;    Malaria;   

DOI  :  10.1186/s12936-015-0835-8

 received in 2015-06-24, accepted in 2015-08-04,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundConcurrent malaria and dengue infection is frequently diagnosed in endemic countries, but its immunopathology remains largely unknown. In the present study, a large panel of cytokines/chemokines and clinical laboratory markers were measured in patients with Plasmodium vivax and dengue co-infection as well as in individuals with malaria or dengue mono-infections in order to identify biosignatures of each clinical condition.MethodsIndividuals from the Brazilian Amazon were recruited between 2009 and 2013 and classified in three groups: vivax malaria (n = 52), dengue (n = 30) and vivax malaria and dengue co-infection (n = 30). P. vivax malaria was diagnosed by thick blood smear and confirmed by PCR; dengue cases were detected by IgM ELISA or NS1 protein. The plasma levels of cytokines and chemokines were determined by multiplex assay.ResultsIndividuals with malaria and dengue co-infection displayed lower levels of platelets and haemoglobin than those with malaria or dengue mono-infections (p = 0.0047 and p = 0.0001, respectively). The group of individuals co-infected exhibited the highest median concentrations of IFN-γ, IL-6, CCL4 than the mono-infected groups. Network analyses of plasma cytokines/chemokines revealed that malaria and dengue co-infection exhibits a distinct immune profile with critical roles for TNF, IL-6 and IFN-γ. Further, parasitaemia levels displayed positive significant interactions with IL-6, CCL4 and IL-10 in the group of patients co-infected with malaria and dengue. No differences were observed in distribution of dengue virus serotypes and Plasmodium parasitaemia levels between the groups.ConclusionsThe findings described here identify unique patterns of circulating immunological markers in cases of malaria and dengue co-infection and provide insights on the immunopathology of this co-morbid condition.

【 授权许可】

CC BY   
© Mendonça et al. 2015