The influence of intestinal parasites on Plasmodium vivax-specific antibody responses to MSP-119 and AMA-1 in rural populations of the Brazilian Amazon

Malaria Journal

Research

Cesarino Junior Lima Aprígio¹  Mauricio Martins Rodrigues²  Irene Silva Soares³  Mariana Pinheiro Alves Vasconcelos⁴  Joffre Rezende Neto⁵  Josue da Costa Lima-Junior⁶  Juan Camilo Sánchez-Arcila⁶  Joseli Oliveira-Ferreira⁶  Virginia Araujo Pereira⁶  Marcelle Marcolino de França⁶  Antonio Têva⁷  Daiana de Souza Perce-da-Silva⁸  Dalma Maria Banic⁸ 

[1] Agencia de Vigilância em Saúde da Secretaria de Estado da Saúde - AGEVISA, Rondonia, Brazil;Centro de Terapia Celular e Molecular (CTCMol), Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil;Departamento de Análises Clínicas e Toxicológicas, Faculdadede Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil;Instituto de Infectologia Emílio Ribas, São Paulo, Brazil;Intituto de Gastroenterologia de Goiânia, Goiânia, Goiás, Brazil;Laboratorio de Imunoparasitologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, Brazil;Laboratório de Imunodiagnóstico, Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública/Fiocruz, Rio de Janeiro, Brazil;Laboratório de Simulídeos e Oncocercose, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;

关键词: Malaria;    Intestinal parasites;    Co-infection;    Plasmodium vivax;    AMA-1;    MSP-1;   

DOI  :  10.1186/s12936-015-0978-7

 received in 2015-04-14, accepted in 2015-10-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPolyparasitism is a common condition in humans but its impact on the host immune system and clinical diseases is still poorly understood. There are few studies of the prevalence and the effect of malaria-intestinal parasite co-infections in the immune response to malaria vaccine candidates. The present study determines whether the presence of malaria and intestinal parasites co-infection is associated with impaired IgG responses to Plasmodium vivax AMA-1 and MSP-119 in a rural population of the Brazilian Amazon.MethodsA cross-sectional survey was performed in a rural area of Rondonia State and 279 individuals were included in the present study. At recruitment, whole blood was collected and Plasmodium and intestinal parasites were detected by microscopy and molecular tests. Blood cell count and haemoglobin were also tested and antibody response specific to P. vivax AMA-1 and MSP-119 was measured in plasma by ELISA. The participants were grouped according to their infection status: singly infected with Plasmodium (M); co-infected with Plasmodium and intestinal parasites (CI); singly infected with intestinal parasites (IP) and negative (N) for both malaria and intestinal parasites.ResultsThe prevalence of intestinal parasites was significantly higher in individuals with malaria and protozoan infections were more prevalent. IgG antibodies to PvAMA-1 and/or PvMSP-119 were detected in 74 % of the population. The prevalence of specific IgG was similar for both proteins in all four groups and among the groups the lowest prevalence was in IP group. The cytophilic sub-classes IgG1 and IgG3 were predominant in all groups for PvAMA-1 and IgG1, IgG3 and IgG4 for PvMSP-119. In the case of non-cytophilic antibodies to PvAMA-1, IgG2 was significantly higher in IP and N group when compared to M and CI while IgG4 was higher in IP group.ConclusionsThe presence of intestinal parasites, mainly protozoans, in malaria co-infected individuals does not seem to alter the antibody immune responses to P. vivax AMA-1 and MSP-119. However, IgG response to both AMA1 and MSP1 were lower in individuals with intestinal parasites.

【 授权许可】

CC BY   
© Sánchez-Arcila et al. 2015