Nascent transcription upon interferon-α2 stimulation on human and rhesus macaque lymphoblastoid cell lines

BMC Research Notes

Data Note

Daniel Ramirez¹  Robin D. Dowell¹  Edward B. Chuong¹ 

[1] Department of Molecular, Cellular, and Developmental Biology; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, USA;

关键词: Homo sapiens;    Macaca mulatta;    PRO-seq;    Type I interferon;    Lymphoblastoid cell lines;   

DOI  :  10.1186/s13104-023-06465-1

 received in 2022-10-04, accepted in 2023-08-18,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectivesThe interferon-triggered innate immune response has been observed to be under strong diversifying selection to counteract the many pathogens hosts have to defend against. In particular, rewiring of gene transcription regulation allows organisms to rapidly acquire new phenotypes by removing and adding genes into the innate immune gene network. Dissecting the molecular processes by which this rewiring takes place, either by changing the DNA regulatory elements or by changing the activity of the regulators across species, is key to better understand this evolutionary process.Data descriptionTo better comprehend the evolutionary dynamics that have occurred in the initial transcriptional response to interferon in primates, we present Precision Run-On (PRO-seq) datasets made after 1 h of interferon-α2 stimulation on human and rhesus macaque lymphoblastoid cell lines. Further, we tested the difference between using either species’ cognate interferon versus using the other orthologous interferon to account for any potential impacts in the interaction of the orthologous interferons with their cellular membrane receptors. This data provides insights into the regulatory mechanisms that drive species-specific responses to environmental perturbations, such as the one driven by the interactions of pathogens and their hosts.

【 授权许可】

CC BY   
© The Author(s) 2023