Identifying human-rhesus macaque gene orthologs using heterospecific SNP probes

Genomics

Jillian Ng²  Sree Kanthaswamy¹  Jessica Satkoski Trask²  Dawei Lin³  Vince S. Buffalo³  Joseph N. Fass³  Cody T. Ross²  David Glenn Smith² 

[1] Molecular Anthropology Lab., Dept. of Anthropology, UC Davis, CA, USACalifornia National Primate Research Center, UC Davis, CA, USADepartment of Environmental Toxicology, UC Davis, CA, USA;Molecular Anthropology Lab., Dept. of Anthropology, UC Davis, CA, USACalifornia National Primate Research Center, UC Davis, CA, USA;Genome Center Bioinformatics Core, UC Davis, CA, USA

关键词: Macaca mulatta;    Single nucleotide polymoprhisms (SNPs);    Homo sapiens;    Heterospecific sequence maps;   

DOI  :  10.1016/j.ygeno.2012.09.001

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

WegenotypedaChineseandanIndian-originrhesusmacaqueusingtheAffymetrixGenome-WideHumanSNPArray6.0andcataloged85,473uniquelymappingheterospecificSNPs.TheseSNPswereassignedtorhesuschromosomesaccordingtotheirprobesequencealignmentsasdisplayedinthehumanandrhesusreferencesequences.Theconservedgeneorder(synteny)revealedbyheterospecificSNPmapsisinconcordancewiththatofthepublishedhumanandrhesusmacaquegenomes.

UsingtheseSNPs'originalhumanrsnumbers,weidentified12,328genesannotatedinhumansthatareassociatedwiththeseSNPs,3674ofwhichwerefoundinatleastoneofthetworhesusmacaquesstudied.Duetotheirdensity,theheterospecificSNPsallowfine-grainedcomparisons,includingapproximateboundariesofintra-andextra-chromosomalrearrangementsinvolvinggeneorthologs,whichcanbeusedtodistinguishrhesusmacaquechromosomesfromhumanchromosomes.

【 授权许可】

Unknown   

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