Effect of exercise on chemically-induced colitis in adiponectin deficient mice

Journal of Inflammation

Research

Emma Fletcher¹  Bianca Larsen¹  Arpit Saxena¹  J Larry Durstine²  Raja Fayad²  Manjeshwar Shrinath Baliga³ 

[1] Exercise Science, Applied Physiology Division, University of South Carolina, Columbia, SC, USA;Exercise Science, Applied Physiology Division, University of South Carolina, Columbia, SC, USA;University of South Carolina, Arnold School of Public Health, 921 Assembly St. room 301, 29208, Columbia, SC, USA;Father Muller Medical College, Kankanady, Mangalore, Karnataka, India;

关键词: Adipokines;    Cytokines;    Inflammation;    Epithelial cell proliferation;    Intestine;   

DOI  :  10.1186/1476-9255-9-30

 received in 2011-05-10, accepted in 2012-06-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundInflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis.MethodsMale adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water.ResultsThe clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED.ConclusionsExercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis.

【 授权许可】

Unknown   
© Saxena et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.