Relationship between the Apolipoprotein AI, B gene polymorphism and the risk of non-traumatic osteonecrosis

Lipids in Health and Disease

Research

Yan-Jie Guo¹  Shi-Chang Zhao¹  Ji-Min Yin¹  Zhong-Tang Liu¹  Zhao Liu² 

[1] Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University, No.600 Yishan Road, 200233, Shanghai, China;Department of hematology, Shanghai Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;

关键词: Apolipoprotein AI;    Apolipoprotein B;    Non-traumatic osteonecrosis;    Gene polymorphism;    Molecular epidemiology;   

DOI  :  10.1186/1476-511X-13-149

 received in 2014-05-18, accepted in 2014-08-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPrevious studies suggested that Apolipoprotein AI (ApoAI) and apolipoprotein B (ApoB) gene polymorphisms may result in lipid metabolism disorders. Genetic polymorphisms in these genes may be associated with the occurrence of osteonecrosis.MethodsWe designed a case-control study including 429 patients of osteonecrosis and 368 age- and sex-matched control subjects. Polymerase chain reaction was used to amplify the DNA fragments in promoter -75 G > A of ApoAI gene and EcoR I, Xba I and 3′-VNTR of ApoB gene in osteonecrosis patients and healthy controls. We utilized polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to genotype these four single nucleotide polymorphisms (SNPs).ResultsFor -75 G > A polymorphism of ApoAI, AA genotype frequency (0.501) was significantly higher in patients with osteonecrosis than that in control (0.462) subjects (P <0.001), GA genotype frequency (0.170) was significantly lower than that in the control (0.310) group (P <0.0001). In osteonecrosis patients, the odds ratio (OR) of A allele was 3.932 (95% CI: 3.0847 ~ 5.0123), which suggested that subjects carrying A allele of promoter region -75 G > A of ApoAI gene had higher susceptibility to osteonecrosis than G allele carriers. The genotype and allele frequency distributions showed no significant difference in EcoR I, Xba Iand 3′-VNTR loci of ApoB gene between the osteonecrosis group and control group.ConclusionOur study suggested that ApoAI gene -75G > A polymorphism may be associated with susceptibility to osteonecrosis in Chinese population. However, our results need further investigation with large sample size and various populations.

【 授权许可】

Unknown   
© Yin et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.